Symrise Ag

Leibniz-Institut Fur Pflanzenbiochemie

Torsten Geissler

Jakob Peter Ley

Gerhard Krammer

Michael Backes

Günter Kindel

Susanne Paetz

Uwe Bornscheuer

Thomas Riess

Ludger A Wessjohann

Kathrin Langer

Johannes Kiefl

Katrin Geissler

Holger Zorn

Jens-Michael Hilmer

Fabia Hentschel

Egon Gross

Martin Dippe

Maren Thomsen

Marco A Fraatz

Johanna Rost

Susann Herrmann

Michael Goldmann

Winfried Hinrichs

Anne-Kathrin Bauer

Morine Verwohlt

Marco Fraatz

The present invention relates to genetically modified enzymes obtained by rational design of the active site binding pocket of the prototypic enzyme 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) for hydroxylating a 4-hydroxyphenyl compound to yield a 3,4-dihydroxyphenyl compound and to biotechnological methods including in vivo and in vitro methods using said enzymes or catalytically active fragments thereof. Further provided is a method either using a suitable oxidase or hydroxylase further enabling the subsequent site specific methylation of the 3,4-dihydroxyphenyl compound in a coupled enzymatic reaction by providing a suitable O-methyltransferase. Finally, compositions obtainable by the aforementioned methods are disclosed.

Mutant 4-hydroxyphenylacetate 3-hydroxylases and uses thereof

A method for producing branched aldehydes is proposed, the method comprising the following steps:

Method for producing branched aldehydes

A method is proposed for producing flavonoids, comprising the steps: (a) providing of a transgenic microorganism, containing (i) a first nucleic acid section (A), comprising or consisting of a gene coding for a CYP450 oxidase, (ii) a second nucleic acid section (B), comprising or consisting of a gene coding for a plant O-methyltransferase, and (b) adding of one or more flavanones to the transgenic microorganism, (c) the conversion of the substrate flavanones by the transgenic microorganism to the corresponding flavonoids, and optionally (d) isolating and purifying of the final products.

Method for the biotechnological production of flavonoids

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Einbeck, Germany

Torsten Geissler