Novozymes A/s

Torben Henriksen, Legal Representative

Martin Schulein

Kim Vilbour Andersen

Claus Von Der Osten

Bo Damgaard

Martin Schulein, Deceased

Carsten Schau Andersen

Johan Smets

Helle Outtrup

Lene Nonboe Andersen

Markus Sakari Kauppinen

Shinobu Takagi

Lene Lange

Thomas Thisted

Soren Flensted Lassen

Mads Eskelund Bjornvad

Keith Gibson

Ruby Ilum Nielsen

Vibeke Skovgaard Nielsen

Michiko Ihara

Lars Hylling Christensen

Mads Eskelund Bjørnvad

Lars Christiansen

Martin Schülein

The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:

Cellulase variants

Detergent composition comprising a surfactant and a pectate lyase (EC 4.2.2.2) enzyme en-coded by a DNA sequence endogeneous to a strain ofor a stabilized variant thereof provides superior cleaning and stain removal.

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The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence and a second amino acid sequence having the following sequence wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

Endoglucanases

The present invention relates to an enzyme exhibiting endo-beta-1,4-glucanase activity (EC 3.2.1.4), which is a) a polypeptide encoded by the DNA sequence of positions 1 to 2322 of SEQ ID NO: 1; b) a polypeptide produced by culturing a cell comprising the sequence of SEQ ID NO: 1 under conditions wherein the DNA sequence is expressed; c) an endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO: 2; and fragments thereof exhibiting endo-beta-1,4-glucanase activity, and d) a polypeptide having endo-beta-1,4-glucanase activity that is encoded by a polynucleotide that hybridizes with the nucleotide sequence shown in positions 1&#x2013;2322 of SEQ ID NO: 1, is useful for detergent and textile applications.

Endo-beta-1,4-glucanases

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Copenhagen, Denmark

Torben Henriksen, Legal Representative