Basf Plant Science Company Gmbh

Basf Plant Science Gmbh

Bioriginal Food & Science Corporation

Toralf Senger

Jörg Bauer

Laurent Marty

Sten Stymne

Jenny Lindberg Yilmaz

Johnathan A Napier

Patricia Vrinten

Carl Andre

Dietrich Rein

Joerg Bauer

Olga Sayanova

Irene Kunze

Richard Haslam

Ruiz Lopez Noemi

Ze Long Lim

Xiao Qiu

Thorsten Zank

Josef Martin Kuhn

Heiko A Haertel

Guohai Wu

John A McElver

Steven Craig Bremmer

Heiko Haertel

Steven D Breazeale

Jorg Bauer

Yiao Qiu

T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.

Materials and methods for PUFA production, and PUFA-containing compositions

Assay method, comprising providing a plant capable of expressing a delta-12 desaturase, wherein said delta-12 desaturase has at least 50% total amino acid sequence identity to at least one of the sequences SEQ ID NO. 328 to 336, and/or at least 59% total amino acid sequence similarity to at least one of the sequences SEQ ID NO. 328 to 336, and wherein the plant is also capable of expressing at least one or more enzymes of unsaturated fatty acid metabolism, of which enzymes at least one is capable of using linoleic acid as a substrate, and of which enzymes at least one is supposedly connected to a plant metabolic property, growing the plant, and measuring said plant metabolic property for said plant.

Stabilising fatty acid compositions

The present invention generally is concerned with the modification of plant lipids containing PUFAs. In this context, the invention is particularly concerned with plants and plant materials for such modifications, wherein the plants preferably are oilseed plants. Regarding plant parts, the invention is particularly concerned with seeds of such plants and preferably seeds of oilseed plants. The invention is also concerned with plant positions obtainable or obtained by the modification method of the invention, and with full stuff of feedstuff comprising such liquid compositions.

Modification of plant lipids containing PUFAs

The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a &#x394;5 and/or &#x394;6 desaturase to the substrate specificity of a &#x394;4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the &#x394;5 and/or &#x394;6 desaturase and (ii) the &#x394;4 desaturase; and replacing in the amino acid sequence of the mentioned &#x394;5 and/or &#x394;6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the &#x394;5 and/or &#x394;6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the &#x394;4 desaturase, thereby converting the substrate specificity of the &#x394;5 and/or &#x394;6 desaturase to the substrate specificity of the &#x394;4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a &#x394;4 desaturase to the substrate specificity of a &#x394;5 and/or &#x394;6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the &#x394;4 desaturase and (ii) the &#x394;5 and/or &#x394;6 desaturase; and replacing in the amino acid sequence of the indicated &#x394;4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the &#x394;4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the &#x394;5 and/or &#x394;6 desaturase, thereby converting the substrate specificity of the &#x394;4 desaturase to the substrate specificity of the &#x394;5 and/or &#x394;6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity.

Methods for conversion of the substrate specificity of desaturases

The present invention provides transgenicevents LBFLFK and LBFDAU and progeny thereof, and cells, seeds, plants comprising DNA diagnostic for these events. The invention also provides artificial oligonucleotide primers and probes that are diagnostic for the LBFLFK and LBFDAU events and their progeny in a sample, and methods for detecting the presence of the LBFLFK and LBFDAU events and their progeny in a sample. The invention further provides oil and commodity products derived from the LBFLFK and LBFDAU events.

events LBFLFK and LBFDAU and methods for detection thereof

The present invention relates to transgenic nucleic acids, expression cassettes, vectors, plant cells, plant organs and plants. The invention also relates to methods for increasing expression or activity of a target gene, particularly in a plant cell or plant organ, and also to uses of recombinant nucleic acids and expression cassettes to increase expression or activity of a target gene or for manufacturing of a vector, plant cell, plant organ or plant. Incidentally, the invention relates to enhancers for achieving increased expression or activity of a target gene, particularly in a plant cell or plant organ, when operably linked to a promoter functional in such plant cell, plant organ or plant. The invention is described herein with reference to the technical field of production of polyunsaturated fatty acids (PUFAs), without being limited to this technical field. For the production of desired molecules in plant cells, e.g. PUFAs, it is frequently required to express a target gene heterologous to the plant cell, or to overexpress a target gene naturally found in said plant cell.

Gene expression or activity enhancing elements

The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

Acyltransferases and uses thereof in fatty acid production

The invention provides nucleic acid molecules which encodes a novel fatty acid desaturase, KCS, KCR and/or LACS fromand. The invention also provides recombinant expression vectors containing the nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA and for screening for delta-4 desaturases.

Enzymes, enzyme components and uses thereof

The present invention pertains to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

The invention provides isolated nucleic acid molecules which encode a fatty acid desaturase, KCS, KCR, DH and ECR from. The invention also provides recombinant expression vectors containing desaturase, KCS, KCR, DH and ECR nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA.

Fatty acid desaturases, elongases, elongation components and uses thereof

The invention in principle pertains to the field of recombinant manufacture of fatty acids. It provides novel nucleic acid molecules comprising nucleic acid sequences encoding fatty acid desaturases, elongases, acyltransferases, terminator sequences and high expressing seed-specific promoters operatively linked to the said nucleic acid sequences wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters.

Regulatory nucleic acid molecules for enhancing seed-specific gene expression in plants promoting enhanced polyunsaturated fatty acid synthesis

The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucletides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

Growing community of inventors

Heidelberg, Germany

Toralf Senger