The Penn State Research Foundation

Other

Duke University

Tony Jun Huang

Xiaole Mao

Hai Huang

Xiaoyun Ding

Stephen J Benkovic

Peiran Zhang

Daniel Ahmed

Shujie Yang

Ahmad Ahsan Nawaz

Jingie Shi

Technologies for harmonic acoustic manipulation of colloidal particles include a system having a piezoelectric substrate coupled to one or more segmented acoustic transducers and a fluid positioned above the substrate. The segmented transducers have multiple segments, each with a resonant frequency equal to a harmonic frequency. The system further includes a controller that generates a harmonic signal including multiple harmonic components and applies the signal to the segmented acoustic transducers to generate an acoustic potential field in the fluid and manipulate the colloidal particles. The system may translate or rotate the particles, and may form the particles into a colloidal crystal monolayer. The system may selectively pair or otherwise group and separate individual particles. The system may pair and separate multiple groups of particles. The system may measure adhesion between particles. The system may pattern particles over a surface. The colloidal particles may be cells.

Technologies for particle manipulation using harmonic acoustic waves

The invention is based on speed changes of sound/ultrasound pulses and a fixed detecting depth between a transducer and sampling points to collect information of the detecting depth and/or a velocity of motionless and/or moving objects from the sampling points to construct two-dimension or three-dimension images of the sampling points. By taking advantages of a pulse ultrasound and a continuous ultrasound, a method of coded sound pulses can simultaneously collect the information of the detecting depth and the velocity from the sampling points, which improves imaging quality. Calculating a speed of the moving objects by simultaneously detecting time-of-flight (TOF) and TOF shift at same site from two separated piezoelectric (PZT) elements improves testing results with accuracy, simplification and reproducibility. An aliasing can be rectified based on the TOF and the TOF shift.

Two dimension and three dimension imaging based on speed changes of sound/ultrasound

Acoustofluidic systems including acoustic wave generators for manipulating fluids, droplets, and micro/nano objects within a fluid suspension and related methods are disclosed herein. According to an aspect, an acoustofluidic system includes a substrate including a substrate surface. The system also includes an acoustic wave generator configured to generate acoustic streaming within an acoustic wave region of the substrate surface. Further, the acoustic wave generator is controllable to change the acoustic streaming for movement of a droplet or other micro/nano object on a fluid suspension about the acoustic wave region.

Acoustofluidic systems including acoustic wave generators for manipulating fluids, droplets, and micro/nano objects within a fluid suspension and related methods

During transmission, a speed of sound pulses gradually reduces due to acoustic impedance. Regulating a length or a density or a sound speed of the sound pulses affects their average speed in the transmitting medium, sound intensity and detecting depth. Time of flight (TOF) and TOF shift can be used to calculate the depth and moving speed of detecting objects. Calculating a speed of moving objects by simultaneously detecting TOF shift at same site from two separated piezoelectric (PZT) elements improves the testing results with accuracy, simplification and reproducibility. Coding sound pulses to obtained the TOF and the TOF shift will simultaneously calculate the depth and the moving speed of sampling points, which can be used to construct 2D and 3D images for these motionless and/or moving sampling points. Coded sound pulses also improves the quality of the imaging.

Two dimension and three dimension imaging with coded pulses based on speed changes of sound/ultrasound

During transmission, a speed of ultrasound pulses gradually reduces due to their energy loss from acoustic impedance. A thickness and a density of piezoelectric (PZT) elements and a sound speed in the PZT elements decides energy of the ultrasound pulses and their detecting depth. A speed of moving objects and an angle of the moving objects with the ultrasound pulses may change a speed of reflected ultrasound pulses and affect their time of flight (TOF) and TOF shift. A method of Coding ultrasound pulses combines advantages of a continuous wave ultrasound and a pulsed wave ultrasound. So, it can be used to obtained the TOF and the TOF shift and calculate the depth and the moving speed of the detecting objects, which also avoids a problem of an aliasing for highly moving speed of the objects.

Calculation of detecting depth and moving speed of objects with coded pulses based on speed changes of ultrasound/sound

Apparatuses and methods for generating a chemical gradient within a flow channel include providing at least one bubble support structure within the flow channel. A bubble support structure helps maintain a bubble at a predetermined location in flow channel when a fluid flow passes therethrough. Oscillations are induced in the bubble using acoustic waves, which may be provided by a piezoelectric transducer located proximate the flow channel. Two or more inlets provide fluids of different chemical compositions into the flow channel, and bubble oscillations are used to generate a dynamically controllable mixing process.

Spatiotemporal control of chemical microenvironment using oscillating microstructures

An apparatus for manipulating particles within a fluid sample includes a substrate having a substrate surface. A surface acoustic wave (SAW) generator generates a SAW within a SAW region of the substrate surface. The SAW has an SAW direction aligned with a pressure node. A channel is configured to receive the fluid sample and the fluid sample has a flow direction which is at an oblique angle to the SAW direction.

High-efficiency separation and manipulation of particles and cells in microfluidic device using surface acoustic waves at an oblique angle

An apparatus for manipulating particles uses tunable standing surface acoustic waves includes a channel defined on a substrate and a pair of variable frequency interdigital transducers. The channel is disposed asymmetrically between the transducers such that the zero order node location is outside of a working region in the channel.

Microfluidic manipulation and sorting of particles using tunable standing surface acoustic wave

A microfluidic device comprises inlets for a sample flow and an out-of-plane focusing sheath flow, and a curved channel section configured to receive the sample flow and out-of-plane focusing sheath and to provide hydrodynamic focusing of the sample flow in an out-of-plane direction, the out-of-plane direction being normal to a plane including the curved channel. Examples of the invention also include improved flow cytometers.

Three-dimensional (3D) hydrodynamic focusing using a microfluidic device

Examples of the present invention include apparatus and methods for particle focusing, for particles within a fluid sample. An example apparatus, which may be a microfluidic device, comprises a substrate, a channel receiving the fluid sample, and at least one surface acoustic wave (SAW) generator. The SAW generator may comprise electrodes supported by the substrate. In some examples, the channel has a particle focusing region located near a region of the substrate surface in which a SAW is generated. Particles are concentrated within one or more particle focus regions of the sample flow (the particle focus regions being appreciably narrower than the channel dimensions) by the effects of the SAW. As an example, a pair of SAW generators can be used to generate a standing surface acoustic wave (SSAW) that is used for particle focusing.

Particle focusing within a microfluidic device using surface acoustic waves

A lens device includes a substrate having a channel and a first fluid flow path and a second fluid flow path. The first and second fluid flow paths at least partially in communication with the channel. A light emitting device is positioned adjacent to the channel. At least one first fluid source is in communication with the first fluid path such that a first fluid is moveable along the first fluid path and at least one second fluid source in communication with the second fluid path such that a second fluid is moveable along the second fluid path. The fluid paths are configured so the first fluid and second fluid move through the channel to define an adjustable liquid gradient refractive index distribution in the channel. Adjustment of the liquid gradient refractive index distribution permits a change of liquid lens focal distance or an angular adjustment of light.

Tunable liquid gradient refractive index lens device

A lens device includes a substrate having a channel and a first fluid flow path and a second fluid flow path. The first and second fluid flow paths at least partially in communication with the channel. A light emitting device is positioned adjacent to the channel. At least one first fluid source is in communication with the first fluid path such that a first fluid is moveable along the first fluid path and at least one second fluid source in communication with the second fluid path such that a second fluid is moveable along the second fluid path. The first second fluid paths are configured so the first fluid and second fluid move through the channel to define an adjustable liquid gradient refractive index distribution in the channel. Adjustment of the liquid gradient refractive index distribution permits a change of liquid lens focal distance or an angular adjustment of light emitted by the light emitting device.

A microfluidic device comprises inlets for a sample flow and an out-of-plane focusing sheath flow, and a curved channel section configured to receive the sample flow and out-of-plane focusing sheath and to provide hydrodynamic focusing of the sample flow in an out-of-plane direction, the out-of-plane direction being normal to a plane including the curved channel.

Growing community of inventors

State College, PA, United States of America

Tony Jun Huang