Promega Corporation

Tonny Johnson

Keith V Wood

Lance P Encell

Monika G Wood

Dieter Klaubert

Mark McDougall

Chad Zimprich

Georgyi V Los

Aldis Darzins

Daniel J Simpson

Roderick G Flemming

Rebecca Godat

Laurie Engel

John William Shultz

Kristopher Zimmerman

Robin Hurst

John W Schultz

Sanchayita Kar

Laura L Bozek

Judith N Stevens

Chad Zimbrich

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule

The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.

Methods for separating molecules

Disclosed are methods of separating a target molecule from a non-target molecule using zinc- or cobalt-charged solid supports.

Methods and kits for purifying his-tagged proteins

Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.

Cell lysis composition, methods of use, apparatus and kit

The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.

Compositions for separating molecules

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Madison, WI, United States of America

Tonny Johnson