Carlsberg A/s

Carlsberg Breweries A/s

Toni Wendt

Ole Olsen

Søren Knudsen

Birgitte Skadhauge

Finn Lok

Katarzyna Krucewicz

Lucia Marri

Hanne Cecille Thomsen

Magnus Wohlfahrt Rasmussen

Massimiliano Carciofi

Soren Knudsen

Ross Fennessy

Alexander Striebeck

Pai Rosager Pedas

Magnus Rasmussen

Christoph Dockter

Hanne Thomsen

Jesper Harholt

Gustav Hambraeus

Jeppe Thulin Østerberg

Sabrina Bodevin

Rasmussen Magnus Wohlfahrt

Hanne Cecilie Thomsen

In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach

The invention relates to barley plants having a high α-amylase activity. The barley plants of the invention may for example carry a mutation in one or more α-amylase promoters, in the HIRT gene, in the HBL12 gene and/or in the WRKY38 gene. The invention further provides plant products prepared from said barley plants.

Barley with increased hydrolytic enzyme activity

The present invention provides methods of preparing plants, with specific predetermined mutation(s) in one or more NOI(s). The specific predetermined mutation(s) preferably may result in the identification of plants having desired traits.

Methods for preparing mutant plants

The present invention relates to methods for preparing cereal-based beverages. The present invention provides, for example, methods for steeping and germination of cereal grain under continuous aeration. Particularly, the present invention provides methods for heating the germinated cereal grains prior to wet milling of germinated cereal grains and direct transfer of the germinated grain, without drying, to the brewery for further processing. Compared to current methods the methods of the present invention significantly reduce water consumption, energy consumption and transport need.

Method for producing an extract of cereal and method for processing this extract into beverage

The present invention relates to methods for preparing cereal-based beverages. Particularly, the present invention provides, for example, methods for steeping and germination of cereal grain under continuous aeration. The present invention also provides for wet milling of germinated cereal grains and direct transfer of the germinated grain, without drying, to the brewery for further processing. Compared to current methods the methods of the present invention significantly reduces water consumption, energy consumption and transport need.

Refined cereal-based beverages

The present invention relates to barley plant or a part thereof, wherein the kernels of said barley plant have a reduced (1,3;1,4)-&#x3b2;-glucan content. The barley plant may carry a mutation in the CslF6 gene, wherein said mutated CslF6 gene encodes a mutant CslF6 polypeptide.

Cereal plants with improved cell wall properties

Growing community of inventors

Frederiksberg, Denmark

Toni Wendt