Monsanto Technology LLC

Toni Armstrong

Larry A Gilbertson

Michael W Petersen

Brian J Martinell

Xudong Ye

Shihshieh Huang

Qi Wang

Kenneth A Barton

Yong Huang

Kenneth John Piller

Laura J Crow

David L DeBoer

The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

Compositions for enhancing segregation of transgenes in plants

Methods for enhancing segregation of transgenes in plants

Methods for the regeneration of cotton plants are disclosed. The use of selective light conditions, novel compositions of media, and solid support matrices during stages of development resulted in increased frequencies of embryogenesis, embryo maturation and embryo germination. The improved process resulted in higher production frequencies of transformation of cotton.

Method for the regeneration of cotton

The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

Methods for enhancing segregation of transgenes in plants and compositions thereof

Growing community of inventors

St. Louis, MO, United States of America

Toni Armstrong