The University of Oklahoma

Tongzhong Ju

Richard D Cummings

William M Canfield

Core 1 &#x3b2;3-galactosyl transferase specific molecular chaperones (Cosmc-1) and nucleic acids encoding the core 1 &#x3b2;3-galactosyl transferase specific molecular chaperones or proteins having core 1 &#x3b2;3-galactosyl transferase specific molecular chaperone activity are described. The polynucleotides encoding Cosmc-1 can be used to transform or transfect host cells for producing substantially pure forms of active forms of core 1 &#x3b2;3-galactosyl transferase and/or for use in an expression system for post-translational core 1 glycosylation of proteins or peptides produced within the expression system, for example, glycosylation via a &#x3b2;3-linkage, of an N-acetylgalactosamine linked to a serine, threonine or other linking amino acid on peptides or proteins requiring O-, N-, or S-linked glycosylation.

Core 1 &#x3b2;3-galactosyltransferase specific molecular chaperones, nucleic acids, and methods of use thereof

Core 1 &bgr;3-galactosyl transferases and nucleic acids encoding the core 1 &bgr;3-galactosyl transferases are described. The enzymes and the nucleic acids encoding said enzymes have been identified in human, rat, mouse and . The polypeptides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational core 1 O-linked glycosylation of proteins or peptides produced within the expression system. The enzymes can be used to galactosylate, via a &bgr;3-linkage, an N-acetylgalactosamine linked to a serine, threonine or other O-linking amino acid on peptides or proteins requiring O-linked glycosylation.

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Edmond, OK, United States of America

Tongzhong Ju