Rubicon Genomics, Inc.

Takara Bio Usa, Inc.

The Methodist Hospital Research Institute

Tong Sun

Vladimir L Makarov

Emmanuel Kamberov

Eric Bruening

Takao Kurihara

Jonathon H Pinter

Irina Sleptsova

Brendan J Tarrier

Tim Tesmer

Joseph M'Mwirichia

Mauro Ferrari

Haifa Shen

Jianhua Gu

Rita Elena Serda

Ismail Mustafa Meraz

Jonathan H Pinter

Xiaojun Xia

Eric E Bruening

Joseph M M'mwirichia

John Pinter

Joseph M M'Mwirichia

Jon Pinter

The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.

Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process

The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries. In particular, the method employs preparation of a DNA molecule by digesting the DNA molecule with at least one methylation-sensitive restriction enzyme; incorporating a nucleic acid molecule into at least some of the digested DNA molecules by either (1) incorporating at least one primer from a plurality of primers that have a 5' constant sequence and a 3&#x2032; variable sequence, wherein the primers are substantially non-self-complementary and substantially non-complementary to other primers in the plurality; or (2) incorporating an oligonucleotide having an inverted repeat and a loop under conditions wherein the oligonucleotide becomes blunt-end ligated to one strand of the digested DNA molecule, followed by polymerization from a 3&#x2032; hydroxyl group present in a nick in the oligonucleotide-linked molecule; and amplifying one or more of the DNA molecules.

Methods and compositions for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation

Disclosed are mesoporous silicon multi-stage vehicles that comprise liposomal-based second-stage particles, as well as pharmaceutical compositions and formulations including such vectors for use in a variety of diagnostic and therapeutic indications. In particular embodiments, MSV comprising ligand decorated second-stage particles are provided for therapeutic methods including, for example, treatment of mammalian cancers, including those of the human breast.

Mesoporous silicon particles for the presentation of tumor antigens and adjuvant for anti-cancer immunity

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Houston, TX, United States of America

Tong Sun