Kabushiki Kaisha Yakult Honsha

Tomoyuki Sako

Saeko Sawaki

Satoshi Matsumoto

Koichi Watanabe

Masahiko Mutai

Masashi Sakai

Shusuke Hashimoto

Koji Miyazaki

Teruo Yokokura

Yasuhisa Shimakawa

Toshizo Sakurai

Tsunekazu Watanabe

Masaki Serata

Junji Fujimoto

Mayumi Kiwaki

Yoshiyuki Shishido

Kisaku Shimura

Mika Miura

Tsuneo Matsumoto

Harue Sone

Kan Shida

Isamu Kondo

Masaharu Onoue

Hiroshi Sansawa

Osamu Matsuo

Yukio Shirasawa

Mariko Kadota

Emi Yasuda

A method for producing bacteria belonging to the genushaving excellent viability even under various conditions with different environmental factors, novel bacteria belonging to the genusobtained by the method, and a method for detecting the bacteria are provided. By subculturing and storing bacteria belonging to the genusalternately in systems under conditions with different environmental factors, the bacteria belonging to the genusexhibiting excellent viability under all the conditions used for the alternate subculturing and storing can be produced.

[object Object]

The invention provides a gene encoding a protein selected from among the following proteins (a) to (c): (a) a protein having any of the amino acid sequences of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, and 108; (b) a protein which has an amino acid sequence equivalent to any of the amino acid sequences of (a), except that one to several amino acid residues are deleted, substituted, or added, and which exhibits cytokine production regulatory activity; and (c) a protein which has an amino acid sequence having 90% or higher identity to any of the amino acid sequences of (a), and which exhibits cytokine production regulatory activity, as well as a gene useful for regulating cytokine production and use of the gene.

Cytokine production regulator gene and use thereof

Methods for transferring a foreign gene into a host chromosome by integrating the foreign gene into a vector by utilizing a lysogenic phage, for the purpose of deleting unnecessary genes derived from the vector, except for the foreign gene. One method comprises (a) preparing a vector with a lysogenic phage integration site (such as an attP site) arranged between a first partial sequence of the objective foreign gene to be transferred, but lacking one terminal region thereof and a second partial sequence of the gene, but lacking the other terminal region, the second partial sequence having an overlapping region with a portion of the first partial sequence; (b) integrating the vector obtained in step (a) into the host chromosome; and (c) screening from among the recombinants obtained in step (b) a recombinant from which unnecessary genes originating in the vector have been deleted owing to the homologous recombination mechanism functioning in the overlapping region between the first and second partial sequences.

Methods for transferring gene into chromosome

Thrombolytic peptide, production thereof, and thrombolytic agent

Novel coliform bacillus useful for industrial production of staphylokinase, a fibrinolytic enzyme, and process for the production of staphylokinase using said microbe are provided. The novel microbe comprises a recombinant DNA, carrying genetic information for the production of staphylokinase, said genetic information being DNA derived from a temperate phage DNA of Staphylococcus aureus.

Coliform bacillus having a gene for the production of staphylokinase and

Growing community of inventors

Minato-ku, Japan

Tomoyuki Sako