Totani Corporation

Nb Health Laboratory Co., Ltd.

Kansai Medical University

Tomoyuki Nakamura

Maretoshi Hirai

Mikio Totani

A heat seal bar is mounted on and removed from a heater unit in a plastic bag making machine without difficulty, An insert is received and fixed to a receiving recess formed in the heat seal bar. A tension pin is supported by the heater unit, inserted into an adjacent recess, and then inserted into an axial groove through an axial opening. The tension pin is pulled and moved axially thereof by an actuator member at the heater unit until a head of the tension pin engages an inward protrusion of the insert. The tension pin and the insert cooperate to mount the heat seal bar on the heater unit.

Heat seal bar mounting apparatus of plastic bag making machine

The present invention provides a technique for conveniently and efficiently regenerating elastic fiber retaining a normal structure, and a technique enabling preparation of an artificial tissue comprising elastic fiber (for example, artificial skin, artificial blood vessels) that can be transplanted to humans. Specifically, the invention provides a method of producing elastic fiber, comprising culturing (for example, culturing in serum-free medium) cells having the capability of regenerating elastic fiber in the presence of DANCE and/or fibulin-4; artificial elastic fiber comprising DANCE and/or fibulin-4; an elastic fiber regenerating agent; a serum-free medium comprising DANCE and/or fibulin-4; and cells having the capability of regenerating elastic fiber, transfected with at least one of a DANCE expression vector, a fibulin-4 expression vector and a DANCE inducing factor expression vector, and the like.

Method of regenerating elastic fiber with the use of dance or factor enhancing the expression thereof

A method of screening for a medicine capable of regulating generation of elastic fibrous tissue; and various means that are requisite for the method. In particular, the invention includes a polypeptide obtained by cleaving DANCE and a polynucleotide coding for the polypeptide; a method of cleaving DANCE; an antibody against the polypeptide obtained by cleaving DANCE; a method of measuring the amount of DANCE cleavage and a kit therefore; a DANCE variant and its polynucleotide; various DANCE complexes and a method of preparing such; a method of screening for a substance capable of regulating DANCE or a DANCE-specific protease, and a substance obtained by such screening method; an agent for regulating generation of elastic fibrous tissue; and a kit with DANCE and a polynucleotide coding for the same.

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Kyoto, Japan

Tomoyuki Nakamura