Toa Medical Electronics Co., Ltd.

Tomoyuki Kuroda

Takashi Sakata

Syouhei Matsuda

Takahito Fukuhara

A reagent and a method for classifying leukocytes with a flow cytometer by means of optical measurements on fluorochrome-stained blood cells are included. The reagent and the method are useful in the practice of clinical testing.

Reagent and method for classifying leukocytes by flow cytometry

A method for classifying leukocytes with a flow cytometer by use of optical measurements on fluorochrome-stained blood cells. The method comprises the steps of lysing erythrocytes in a blood sample, selectively staining eosinophils and basophils, permitting stained sample to flow through a flow cytometer, and identifying and counting the type of each leukocyte.

Method for classifying leukocytes by flow cytometry

Three methods for classifying leukocytes with a flow cytometer by optical measurements on fluorochrome-stained blood cells or those which have been so treated as to provide varying intensity in forward scattered light are useful in the practice of clinical testing. Also included are reagents employed in the practice of such methods.

Method of classifying leukocytes by flow cytometry and reagents used in

A reagent for classifying leukocytes with a flow cytometer by means of optical measurements on fluorochrome-stained blood cells is included. The reagent is useful in the practice of clinical testing and includes a Neutral Red fluorochrome and either an Astrazon Organe G or an Auramine O fluorochrome to enable differentiation of leukocytes.

Reagent for classifying leukocytes by flow cytometry

Reagents used in a method of classifying leukocytes by flow cytometry

Disclosed is a reagent containing auramine O for the fluorescent staining of reticulocytes in a sample of whole blood to permit quantitative determination of the reticulocytes by means of a flow cytometer.

Reticulocyte quantitating reagent for flow cytometry

A reagent for reticulocyte counting by flow cytometry which comprises two solutions, namely, a stock solution for staining in which a dye is dissolved in a nonaqueous solvent, and a buffer solution which satisfies the optimum staining conditions. By combining these two solutions immediately before measurement, a stable final staining solution for reticulocyte counting can always be obtained.

Reagent for reticulocyte counting by flow cytometry

A reagent for reticulocyte counting by flow cytometry characterized in that it contains a carbonate salt. If a blood sample is left exposed to ordinary air, non-specific staining of erythrocyte can be prevented with the addition of not less than about 1 mM of NaHCO.sub.3.

A method for classifying leukocytes with a flow cytometer by means of optical measurements on fluorochrome-stained blood cells is useful in the practice of clinical testing.

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Kakogawa, Japan

Tomoyuki Kuroda