Keio University

Tohoku University

National University Corporation National Higher Education and Research System

Salivatech Co. , Ltd.

Tomoyoshi Soga

Hiroshi Suzuki

Makoto Suematsu

Akiyoshi Hirayama

Takaaki Abe

Shinichi Akiyama

Shoichi Maruyama

Hiroshi Abe

Makoto Sunamura

Masashi Honma

Takehito Yamamoto

Masahiro Sugimoto

An object of the present invention is to provide a method capable of detecting whether a subject has systemic lupus erythematosus (preferably nephrotic syndrome) with high accuracy without performing a renal biopsy, a biomarker for carrying out such detection, and the like. The concentration of 3',4′-didehydro-3′-deoxycytidine in urine collected from a test subject is measured, and this concentration is compared with a reference concentration for control, which allows to detect whether the above-mentioned test subject has systemic lupus erythematosus (preferably lupus nephritis) with high accuracy.

Method for detecting SLE

Processing a capillary distal end into acute-angle, an electrophoretic liquid passing hole through which electrophoretic liquid pass is opened in a flexible insulating plate. An electrodialysis-membrane is bonded covering the electrophoretic liquid passing hole; the capillary is securely bonded to the insulating plate portion with no gap therebetween, the portion excluding electrophoretic liquid passing hole on the electrodialysis top-membrane. A crack forms at the capillary portion at the electrophoretic liquid passing hole, with the capillary entirely secured to the insulating plate except portion at the electrophoretic liquid passing hole. The capillary is securely bonded to the insulating plate; reservoir stores electrophoretic liquid on the insulating plate-side to which the capillary is unsecured. An electrode insertion hole into which an electrode is inserted opened in the reservoir upper portion; the electrode is securely inserted into the electrode insertion hole. By this, high-sensitivity measurement is using a spray device for sheathless CE-MS.

Method of creating spray device for sheathless CE-MS, spray device for sheathless CE-MS, and sheathless CE-MS device

The present invention relates to a method for diagnosing, and treating renal disease in a patient by having a test performed for detecting or quantifying one or more renal disease markers present in a test blood sample from the patient; and administering treatment to improve renal function. In particular embodiments, the test performed quantifies cis-aconitate, and the patient is identified as having the renal disease when a concentration of cis-aconitate present in the patient's test blood sample is higher than that of a control. Methods of the present invention can allow diagnosis and treatment of patients with early stage renal disease, such as early stage renal failure. Another aspect of the present invention relates to methods for screening for a prophylactic/therapeutic agent for treating renal disease using one or more renal disease markers.

Human renal disease marker substance

Provided is a biomarker that enables easy and rapid detection of oxidative stress on a living organism and enables prevention of tissue damage or cell necrosis by drug administration, and which is a powerful marker for the study of toxicity and pharmacokinetics of various agents. Oxidative stress is determined by measuring blood concentration of ophthalmic acid, which is a substance that varies in blood depending on the variation of reduced glutathione (GSH) concentration in a biological sample with the use of an analyzer such as a capillary electrophoresis-mass spectrometer. Further, an anti-oxidative stress agent is screened by administering an anti-oxidative stress candidate agent to a non-human animal under oxidative stress conditions, measuring blood concentration of ophthalmic acid, and evaluating the degree of decrease in the ophthalmic acid concentration.

Method for determination of oxidative stress

Growing community of inventors

Yamagata, Japan

Tomoyoshi Soga