National Institute of Advanced Industrial Science and Technology

Kureha Corporation

Toyobo Co., Ltd.

Toyo Boseki Kabushiki Kaisha

Jnc Corporation

Sumitomo Forestry Co., Ltd.

Tomotake Morita

Azusa Saika

Atsushi Sogabe

Shuhei Yamamoto

Dai Kitamoto

Tokuma Fukuoka

Akinori Matsushika

Masahiro Watanabe

Tomohiro Sugahara

Yuusuke Nakamichi

Hironaga Akita

Masaru Kitagawa

Michiko Suzuki

Tomohiro Imura

Tatsuyuki Koshiyama

Shun Sato

Kotaro Ino

Hideaki Koike

Kouichi Ohshiman

Tomo Kakitani

Kazunori Ushimaru

Yukihiro Higashiyama

Mutsumi Kaneko

Provided is a method for efficiently producing a monoacyl MEL. The method comprises culturing a monoacyl-MEL-producing microorganism in the presence of a surfactant.

Production of monoacyl MEL

Provided is a means for increasing mannosylerythritol lipid (MEL) production efficiency. The present invention is a mannosylerythritol-lipid-producing microorganism transformed with an expression vector having a gene that encodes a lipase under the control of E5Pgap promoter or E5Ptef promoter.

Biosurfactant-producing recombinant microorganism

The purpose of the present invention is to provide a PHA, in particular PHBV, having a molecular weight exceeding 3,610,000 and/or a method for producing such a PHA while controlling the molecular weight thereof. Provided is a copolymer of random copolymerization of 3-hydroxybutanoic acid and 3-hydroxyvaleric acid, having a weight-average molecular weight exceeding 3,610,000.

Technique for controlling molecular weight of PHA copolymer produced by halobacterium

A microorganism of the present invention is(NITE BP-03054),(NITE BP-03051),(NITE BP-03052), or(NITE BP-03053).

Method for producing novel microorganisms and ergothioneine

There is provided a novel ionic composite material that can be molded into various shapes using lignin sulfonic acid as one of raw materials thereof, and having flexibility and elasticity, to significantly improve the strength and toughness and impart complete biodegradability thereto. It was found that a combination of &#x3b5;-polylysine (&#x3b5;-PL) which is a cationic polymer that is produced by microorganisms and lignin sulfonic acid exhibits excellent strength and toughness. In addition, &#x3b5;-PL used in this technology is a biodegradable polymer that is completely degraded by microorganisms and the like in the environment. Since lignin sulfonic acid is also a biodegradable polymer, it is thought that a complex in which &#x3b5;-PL and lignin sulfonic acid are mixed in this technology will exhibit complete biodegradability, and more applications thereof can be expected when utilizing the strength, durability, and biodegradability thereof in addition to the improved strength and toughness.

Ionic composite material including lignin sulfonic acid and E-polylysine as components

An enzyme having the following characteristics (a) and (b):

D-type amino acid dehydrogenase

An enzyme has an activity of reversible dehydrogenation of D-amino acid and is a hexamer of polypeptides having an amino acid sequence that has 80% or more identity with the amino acid sequence of SEQ ID NO: 2. The amino acid sequence of the polypeptide can include one or more amino acid substitutions for one or more amino acid residues of SEQ ID NO: 2.

The present invention includes as an active ingredient at least one biosurfactant, in particular mannosyl alditol lipid (such as MEL and MML) or triacylated mannosyl alditol lipid. This allows providing an activator and anti-aging agent that is excellent in activating and anti-aging effects on cells and that is safe enough to be used for a long time, and also providing cosmetics, quasi-drugs, drugs, and drinks and foods including the activator and the anti-aging agent as active ingredients. Further, the present invention provides MEL whose mannosyl erythritol skeleton in a molecular structure is 1-O-&#x3b2;-D-mannopyranosyl-meso-erythritol and a method for producing the MEL with use of a microorganism.

Activator including biosurfactant as active ingredient, mannosyl erythritol lipid, and production method thereof

Growing community of inventors

Tsukuba, Japan

Tomotake Morita