Kumiai Chemical Industry Co., Ltd.

Tohoku University

National Institute of Advanced Industrial Science and Technology

Tomonori Fujioka

Masayuki Machida

Kiyoshi Kawai

Takashi Kubo

Shigenari Yamaguchi

Keietsu Abe

Akira Yoshimi

Maiko Umemura

This invention is intended to identify a gene cluster involved in biosynthesis of a cyclic peptide compound produced by a filamentous fungus of thespecies and to establish a system for synthesizing such cyclic peptide compound. The gene is composed of a first module to a tenth module and encodes a protein having activity of synthesizing a nonribosomal peptide constituting a basic peptide backbone of a cyclic peptide compound produced by a filamentous fungus of thespecies.

Gene involved in synthesis of cyclic peptide compound, method for producing cyclic peptide compound using the same, and transformant comprising the same

An object of the present invention is to identify an enzyme having activity of synthesizing dihydrotentoxin that is a tentoxin precursor and an enzyme having activity of synthesizing tentoxin using dihydrotentoxin as a substrate. The present invention concerns a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 16 and having activity of nonribosomal peptide synthesis of dihydrotentoxin and a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 18 and having activity of converting dihydrotentoxin to tentoxin.

Method for producing dihydrotentoxin

Tentoxin synthesis gene, a method for producing tentoxin or dihydrotentoxin using the same, and a transformant comprising the same

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Tokyo, Japan

Tomonori Fujioka