Shimadzu Corporation

Tomoko Yamaguchi

Hideshi Fujiwake

Natsuki Iwata

A sample is dissolved in a reagentcontaining an organic solvent in a conversion vessel. A gas is supplied from a first gas supply part into the conversion vesselvia a reagent introduction tube, and thus the interior of the conversion vesselis pressurized. A gas is supplied from a second gas supply part into the reagentin the conversion vesselvia a reagent discharge tube, and thus gas bubblesare formed in the reagent

Device for pretreatment of sample and analyzer equipped therewith, and method for pretreatment of sample

A solution containing double stranded nucleic acid is heated, thereby dissociating the strands, which in turn are cooled and rebounded. A homoduplex and a heteroduplex are formed when the double stranded nucleic acid is a hetero body, while only a homoduplex is formed when the double stranded nucleic acid is a homo body. The temperature of the solution is increased while measuring ultraviolet absorption, thereby acquiring a thermal melting profile. When the heated and cooled solution contains only a homoduplex, the thermal melting profile has a single Tm temperature or two Tm temperatures close to each other. When the heated and cooled solution contains a homoduplex and a heteroduplex, the thermal melting profile has two separate Tm temperatures or further single Tm temperatures close to each other. The method therefore allows for the detection of a heteroduplex or a mutation in a sample double stranded nucleic acid.

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Kyoto, Japan

Tomoko Yamaguchi