Kuraray Co., Ltd.

Public University Corporation Yokohama City University

Tomoko Kosaka

Motohiro Fukuda

Go Tazaki

Hideki Taniguchi

Hitoshi Tsuruta

Yun-Wen Zheng

Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.

Cell culture of fetal liver in layered state in a partitioned micro-space

A cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space are provided. A cell culture chamber ()according to the present invention is a cell culture chamber ()including a plurality of microchambers () formed on a surface thereof, characterized in that convex portions (side walls) that partition the microchambers () adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (), and an intercellular network can be constructed effectively.

Cell culture container and cell culture method

Growing community of inventors

Tsukuba, Japan

Tomoko Kosaka