Suntory Holdings Limited

Amano Enzyme Inc.

Suntory Limited

Tomoko Fujimura

Yoshihiro Nakao

Toshihiko Ashikari

Yukiko Kodama

Keita Okuda

Norihisa Nakamura

Ikuma Mizuguchi

Naoki Matsumoto

Takako Inui

Toru Katase

Yuko Kuritani

Tomoyuki Nakahama

Takakiyo Obara

An object of the present invention is to provide an enzyme preparation useful for reducing purine bodies, especially, in beer or beer-based beverages, and use thereof. Provided is a nucleosidase preparation having an activity ratio (U/U), which is guanine deaminase activity per nucleosidase activity, of 0.4 or less.

Nucleosidase agent having reduced contaminant activity

It is an object of the present invention to provide a novel enzyme useful for producing low-purine foods or beverages. There is disclosed a nucleosidase comprising an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 85% or more identity with the amino acid sequence, or an amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having 88% or more identity with the amino acid sequence.

Nucleosidase

Provided herein are a method for selecting a gene participating in the desired brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of; and (D) analyzing the selected gene.

Screening method for genes of brewing yeast

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Tokyo, Japan

Tomoko Fujimura