Wako Pure Chemical Industries, Inc.

Kanagawa Prefectural Hospital Organization

Fujifilm Wako Pure Chemical Corporation

Fujifilm Corporation

Hirosaki University

Tomokazu Ishikawa

Kenji Nakamura

Hiromi Kumon

Tatsuo Kurosawa

Tohru Yoneyama

Chikara Ohyama

Yuki Tobisawa

Shoichi Matsukuma

Mutsuhiro Date

Koichiro Wada

The present invention relates to an oligonucleotide, comprising a part or the entire of the nucleotide sequence shown in any of SEQ ID NO: 1 to SEQ ID NO: 15, or a part or the entire of the sequence complementary to the nucleotide sequence shown in any of SEQ ID NO: 1 to SEQ ID NO: 15, wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of() gene; a primer or a probe for the detection ofwhich comprises said oligonucleoride; and a method for detection ofusing said primer and/or the probe.

[object Object]

The invention provides a method for detection of mutant-type DNA or/and wild-type DNA by contacting at least one of a single-stranded DNA having a substituted nucleotide, a deficient nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA corresponding to the mutant-type DNA (wild-type DNA) with a probe hybridizing with both single-stranded DNAs, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with the wild-type DNA (wild-type hybrid) (at least one of the obtained mutant-type hybrid and wild-type hybrid has a loop structure), (2) contacting the obtained mutant-type hybrid or/and wild-type hybrid with an intercalator, and (3) detecting the presence or absence of the mutant-type DNA or/and the wild-type DNA by separating the conjugate of mutant-type hybrid and intercalator or/and the conjugate of wild-type hybrid and intercalator.

Method for identification and detection of mutant gene using intercalator

The present invention relates to a method for detecting of a mutant DNA using a probe, comprising:

Method for detecting mutant DNA

The object of the present invention is to provide a novel primer for use in the detection of(), and a method for detection ofconveniently, rapidly and with high precision by using the primer. More specifically, disclosed are an oligonucleotide which comprises a part or an entire of the nucleotide sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:130, SEQ ID NO:131, SEQ ID NO:132, SEQ ID NO:133, SEQ ID NO:134, SEQ ID NO:135 and SEQ ID NO:136, or a part or an entire of the sequence complementary to the nucleotide sequence, and which is capable of hybridizing with the nucleotide sequence for agene; a primer and a probe for use in the detection of, which comprises the oligonucleotide; and a method for detection ofusing the primer and/or probe.

The present invention relates to an oligonucleotide which is designed on the basis of a nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 2 and hybridizes with the endogenous plasmid gene of, oligonucleotide primer and probe for detecting, the detection method ofusing said primer and probe, and a primer for detectingof said oligonucleotide or the use to a probe design.

Primer and probe for detecting chlamydia trachomatis, and method for detecting chlamydia trachomatis using same

Disclosed is a method for detecting mutation(s) in nucleotide sequence, which comprises performing a nucleic acid amplification reaction by using an oligonucleotide or a salt thereof as a primer and a nucleic acid in a sample as a template and detecting a reaction product, wherein the oligonucleotide is so modified at the nucleotide at the second position from the 3'-terminus as to inhibit the nucleic acid synthesis. Also disclosed is a kit for the method. According to the present invention, since it is possible to completely eliminate any false positive result in the determination and correspond to various mutation patterns by a single run of PCR1 reaction, it becomes possible to design a drug-resistance determination system, which can detect possible plural genetic mutations by a single run of multiplex PCR.

Method for detection of mutant gene

Primers and probes are provided for selectively detecting tubercle bacillus, and methods for detecting low copy numbers of human tubercle bacillus () using the same are presented. Oligonucleotides comprising a part of or an entire sequence of the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:S, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, or a part of or an entire sequence of the complementary sequence thereof are disclosed, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in. Primers and probes containing such oligonucleotides, and methods for detectingusing the primers and the probes are presented.

Probe and primer for tubercle bacillus detection, and method of detecting human tubercle bacillus therewith

The present invention discloses an oligonucleotide which comprises a part or the entire sequence of the nucleotide sequence depicted in SEQ ID NO: 1, 2, 3 or 4, or a part or the entire sequence of a nucleotide sequence complementary to the nucleotide sequence, wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of; a primer and a probe for use in the detection ofcomprising the oligonucleotide; and a method for detectingusing the primer and/or probe. The method for detectingenables the detection ofmore rapidly and with higher accuracy compared with a conventional bacterium identification method performed by culture examination on a bacterium. Further, the method can exclude any false positive result for the diagnosis and can also detect and diagnosewith higher accuracy compared with a diagnosis method performed by PCR using a conventional primer and/or probe. Still further, the method can quantify acell.

The present invention discloses an oligonucleotide which comprises a part or the entire sequence of the nucleotide sequence shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8, or a part or the entire sequence of a sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8, wherein the oligonucleotide is capable of hybridizing with a nucleotide sequence ofgene; a primer or a probe for the detection of, which comprises the aforementioned oligonucleotide; and a method for detection ofusing the aforementioned primer and/or the probe. According to the detection method of the present invention, any false-positive result in diagnosis can be eliminated and detection or diagnosis ofcan be carried out with higher accuracy, more preciseness, and more specifically compared to a conventional diagnostic method employing a cell culture assay or a PCR assay. The method also enables to quantify a microbial cell.

An object of the present invention is to provide a novel primer and prove for detecting tubercle bacillus capable of avoiding false positive, and an easy-to-use, rapid and high-sensitivity method for detecting human tubercle bacillus () using the same. The present invention relates to an oligonucleotide comprising a part of or an entire sequence of the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, or a part of or an entire sequence of the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in, a primer and a probe containing the oligonucleotide for detecting, and a method for detectingusing the primer and the probe.

Growing community of inventors

Hyogo, Japan

Tomokazu Ishikawa