Osaka University

Japan Science and Technology Agency

Quantum Biosystems Inc.

Shin-Etsu Chemical Co., Ltd.

Aipore Inc.

Kawasaki Jukogyo Kabushiki Kaisha

Hitachi Maxell, Limited

Nagoya University

Kabushiki Kaisha Toshiba

Minolta Company, Ltd.

Agency of Industrial Science and Technology

Tokyo Institute of Technology

Kyushu University

Tokyo Medical and Dental University

Nomura Micro Science Co., Ltd.

Tomoji Kawai

Masateru Taniguchi

Hitoshi Tabata

Makusu Tsutsui

Kazumichi Yokota

Takuya Matsumoto

Ikuo Fukui

Shichio Kawai

Yoshinobu Baba

Takao Yasui

Junzo Fujioka

Takashi Washio

Noritada Kaji

Takahito Ohshiro

Osamu Murata

Hidekazu Tanaka

Mark F Oldham

Eric S Nordman

Mikio Kishimoto

Yoichi Otsuka

Yasuhisa Naitoh

Takeshi Yanagida

Shunichi Minakata

Fumihiko Yamada

Hideyuki Kawaguchi

Eriko Mizuno

Takeshi Yoshida

Akihide Arima

Soh Ryuzaki

Wataru Tonomura

Masayoshi Tanaka

Makoto Kinoshita

Shunichi Hayamizu

Hiroshi Yoshida

Shinji Matsui

Ichiro Matsubara

Sadato Hongo

Yuji Miyahara

Yukio Nishiyama

Hiroshi Yamashita

Teruo Kanki

Yasushi Hotta

Yoshiharu Ohta

Mina Okochi

Satoyuki Kawano

Toru Ogura

Hideo Tanigawa

Toshio Kawahara

Yukichi Horiguchi

Naoki Asakawa

Yoicho Otsuka

Kentaro Doi

Akira Ishii

Kiyoshi Betsuyaku

Yuji Muraoka

Mao Fukuyama

Hirotoshi Yasaki

Toshihito Ohtake

Ken-ichiro Nakamatsu

Hisao Satoh

Tetsuya Nakazumi

Akira Ishi

Ken-Ichiro Nakamatsu

A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

Number analyzing method, number analyzing device, and storage medium for number analysis

Provided is a channel device that is capable of increasing the concentration of fine particles in a liquid only by use of fluid-dynamic flows without relying on electrostatic interactions. A channel device () in accordance with an embodiment of the present invention includes: a main channel () configured to allow a liquid containing fine particles to flow therethrough; a chamber () that is provided at an end of the main channel () and that is configured to store target fine particles which have increased in concentration; and a side channel () that is connected to a side face of the main channel () and that is configured to allow unwanted liquid to drain therethrough, wherein at least one of a height and a width of the side channel () is smaller than a particle size of the fine particles.

Channel device and method for concentrating fine particles

It is an object to improve detection accuracy of an object as compared with prior arts. A flow passage () provided in a detection device () includes a substrate () and a covering member () provided at a position corresponding to the substrate (). A covering member opening (HL) of the covering member () is provided such that a substrate opening (HL) of the substrate () is not covered with the covering member (). The covering member () is arranged onto the substrate () such that a substrate capacitance and a covering member capacitance are connected in series. The covering member capacitance is lower than the substrate capacitance.

Flow passage

In a method for analyzing samples involving the use of a device for analyzing samples, the device for analyzing samples includes at least a movement part through which a sample moves, and a measurement unit that is formed in a middle of the movement part and that measures a value of an ion current when the sample passes through the movement part. The analysis method includes at least a measurement step for measuring the value of the ion current when the sample passes through the movement part, and a determination step for determining a change over time in a quantity of ions from the value of the ion current measured in the measurement step. The quantity of ions includes a quantity of ions that have leaked from the sample during movement of the sample through the movement part.

Method for analyzing samples and device for analyzing samples

A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.

Device for biological material detection, detection apparatus for biological material detection, method for measuring ion current, and method for identifying biological material

The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.

Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide

Provided is a device for electrical measurement designed to be able to perform high sensitivity detection by reading not only changes in steady-state current, but also the occurrence of transient current, and an electrical measurement apparatus including the device for electrical measurement. The device for electrical measurement includes a substrate on which are formed at least a sample separation channel and a sample migration channel, as well as a sample measuring unit, with one end of the sample separation channel formed to connect to one end of the sample migration channel, and the sample measuring unit including a first measuring unit connected to the sample migration channel, and a second measuring unit connected to the sample migration channel from the reverse side to the first measuring unit.

Device for electrical measurement and electrical measurement apparatus

Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

Biomolecule sequencing devices, systems and methods

According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t&#x3c;a &#x3c;d&#x2266;100or (b) s&#x3c;L, s&#x3c;d&#x2266;100, t&#x3c;L and t&#x3c;d, wherein a, L and s are the diameter, length and width of the target, d is the diameter of the pore, and t is the thickness of the membrane in the proximity to the pore.

Single particle analyzer and single particle analysis method

The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.

Sample analysis method

The present invention provides a method and apparatus for controlling the moving speed of a substance, both of which can adjust the moving speed of a substance to a desired speed. The control method and control apparatus cause a substance to pass through an internal space, in which an electro-osmotic flow is generated, of a surround electrode formed so as to surround part of the moving path of the substance, whereby the control method and control apparatus change the moving speed of the substance.

Method for controlling substance moving speed and apparatus for controlling the same

A method for performing micro fabrication includes using, as a photomask, a self-organizing material-patterned substrate which is soluble in an organic solvent. A method for emitting light includes emitting the light in a pattern of a nucleic acid which is a self-organizing material immobilized on a self-organizing material-patterned substrate. An immobilization layer containing a binding material capable of binding to a self-organizing material is formed on a substrate. Then this immobilization layer is patterned by transferring a protrusion and recess pattern formed in a mold thereto by the imprint process. The self-organizing material is supplied onto the side having the protrusion and recess pattern of the immobilization layer transferred thereto. Thus, the self-organizing material is immobilized according to the protrusion and recess pattern of the immobilization layer owning to the self-organizing ability of the material per se and the binding ability of the binding material contained in the immobilization layer.

Method of patterning self-organizing material, patterned substrate of self-organizing material and method of producing the same, and photomask using patterned substrate of self-organizing material

Growing community of inventors

Osaka, Japan

Tomoji Kawai