Kaneka Corporation

National Hospital Organization

Kyoto University

Tomohisa Kato

Yonehiro Kanemura

Tomoko Shofuda

Hayato Fukusumi

Koji Takahashi

Jun Tomono

Shigehiko Miyamoto

Haruhisa Inoue

Kazuya Hamada

Takayuki Kondo

A liquid injection method for injecting a liquid into a culture vessel includes tilting the culture vessel around a horizontal axis at a tilt angle (X&#xb0;) of greater than 0&#xb0; and 50&#xb0; or less, wherein adherent cells are adhered to the culture vessel; and injecting the liquid into the culture vessel at a predetermined linear velocity (Y mm/s) via a wall surface of the culture vessel tilted at the tilt angle (X&#xb0;), wherein the tilt angle (X) and the linear velocity (Y) satisfy the following (formula 1): Y&#x2264;5.075X+123 (formula 1).

Liquid injection method

This invention relates to a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.

Method for screening for pluripotent stem cell growth-promoting factor

It is an object of the present invention to provide a cell culture medium capable of enhancing cell growth efficiency without using feeder cells, in particular which does not comprise serum. The present invention provides a cell culture medium which comprises growth arrest-specific 6 (GAS6) and does not comprise serum.

Cell culture medium and culture method using the same

A method and a device or kit for detecting a nucleic acid, which enable simple and precise visual detection of a nucleic acid amplified by an nucleic acid amplification method, without necessity of special devices are provided. The method for detecting a nucleic acid in a sample comprises: contacting a sample with a dye to react with each other; and observing a substance produced by the reaction with visible light, and evaluating the presence or absence of a nucleic acid by eye. The device or kit for detecting a nucleic acid in a sample comprises: a carrier that holds a dye which can bind to a nucleic acid; a path for passing a sample through the carrier; and an evaluation part for observing a substance produced by the reaction between the sample and the dye with visible light, and evaluating the presence or absence of a nucleic acid by eye.

Method for detecting nucleic acid, and device or kit

Growing community of inventors

Hyogo, Japan

Tomohisa Kato