Hitachi, Ltd.

Gunma University

Tomoharu Kajiyama

Hideki Kambara

Kunio Harada

Yuji Miyahara

Katsuji Murakawa

Kenji Yasuda

Kazunori Okano

Chihiro Uematsu

Takashi Irie

Hiroko Matsunaga

Masataka Shirai

Masayasu Kuwahara

Kiyomi Taniguchi

Toru Habu

To provide a means and a method of quickly sampling a tissue fragment from a plant tissue, quickly preserving a gene expression state within the tissue fragment, and comprehensively analyzing the gene expression state. The present invention relates to a method of sampling a plant tissue section, comprising: inserting a first gel layer into a needle; arranging a plant tissue on a second gel layer; and passing the needle through the plant tissue together with the second gel layer, and sampling a section of the plant tissue in the needle.

Plant tissue sampling method and plant gene analysis method

This invention provides a nucleic acid amplification device whereby the abundance of a target molecule can be maintained before and after a step of separately amplifying a sample such that highly accurate analysis results that can be applied to gene expression analysis can be obtained. Also, a nucleic acid amplification device having a structure in which a plurality of minute reaction cells each comprising a set of a bead-retaining space capable of retaining a single analysis bead and a reagent reaction space retaining no bead but having a volume that is large enough to cause a reagent reaction therein are positioned so as to form a planar face is provided.

Nucleic acid amplification device

Provided is a nucleic acid substrate which has nucleic acid substrate characteristics equivalent to those of dATP, has a low substrate specificity for luciferase, exerts no negative effect on enzymatic reactions such as a complementary-strand synthesis, and therefore is particularly suitable for the pyrosequencing method. As a nucleic acid substrate complementary to nucleotide T, a 7-substituted deoxyribonucleotide triphosphate whose 7-position of a purine group is modified by a substituent is used as a substitute for a nucleotide &#x3b1;-thiotriphosphate analog.

Method and reagent for gene sequence analysis

It is an object to provide a method of suitably analyzing the amount of gene expression of a single-cell. A method of detecting a nucleic acid comprising

Methods for quantitative cDNA analysis in single-cell

By the conventional technique for dispensing more than one reagents accurately, the system is complicated and thus a compact and inexpensive system is difficult to realize. In the present invention, the pressurized dispensing system utilizing a capillary is realized, and in addition, in order to reduce the leakage of reagents different from the reagent dispensed, by forming air layers at the tips of the capillaries after dispensing, a compact, simple, inexpensive analysis apparatus is realized.

Small size gene analysis apparatus

Reagent container

A biochemical reaction detection chip capable of controlling the temperature for biochemical reactions including hybridizations and its substrate. The function of the chip is performed by comprising a plurality of islands of a heat conducting material on the membrane of the substrate, the islands being spaced from each other and individually provided with temperature controllers, and the probes immobilized on the substrate.

Biochemical reaction detection apparatus

There are beforehand prepared a monomer having a reaction residue and a polynucleotide probe set comprising plural kinds of polynucleotide probes having a residue bonded to the reaction residue. The monomer is mixed with each kind of polynucleotide probes comprising any plural probes selected from the polynucleotide probe set. Each kind of the resultant mixtures is added to each of different small holes to make the mixture into gel matrix. Thus, a polynucleotide probe chip is produced. Sample DNA is forcibly migrated in the gels by electrophoresis. Laser light is projected onto the side face of the chip. The fluorescence emitted from the whole surface of the chip is collectively detected with a high-sensitive two-dimensional detector. Thus, the polynucleotide probe chip, holding various kinds of DNA probes, for detecting DNA can be provided. This chip has high hybridization-efficiency and makes high-sensitivity and high-speed DNA detection possible.

Polynucleotide chip probe and polynucleotide detection method

Advanced thermal gradient DNA chip (ATGC), the substrate for ATGC, method for manufacturing for ATGC, method and apparatus for biochemical reaction, and storage medium

Advanced thermal gradient DNA chip (ATGC) the substrate for ATGC, method for manufacturing for ATGC method and apparatus for biochemical reaction and storage medium

Growing community of inventors

Higashiyamato, Japan

Tomoharu Kajiyama