Daiichi Sankyo Company, Limited

Keiichi Fukuda

Tomofumi Tanaka

Uichi Koshimizu

Hideyuki Okano

Shinsuke Yuasa

Keijiro Sugimura

Takuya Shimazaki

Kayoko Kawashima

Michinori Kadokura

The present invention provides a method for inducing differentiation of cardiomyocytes efficiently and selectively from stem cells. A method for inducing differentiation of cardiomyocytes from pluripotent stem cells, which comprises: (i) culturing the pluripotent stem cells in a culture medium containing no substance that promotes activation of the canonical Wnt signaling pathway during the time period between initiation of differentiation induction and 24 hours before the period of elevated canonical Wnt gene expression; and then (ii) culturing the pluripotent stem cells in a culture medium containing a substance that promotes activation of the canonical Wnt signaling pathway during a time period of 24 to 96 hours, starting from 24 to 0 hours before the period of elevated canonical Wnt gene expression.

Method for inducing differentiation of pluripotent stem cells into cardiomyocytes

For a method of inducing differentiation of cardiomyocytes from stem cells, a method is provided to induce efficiently and selectively differentiation of cardiomyocytes by such a method in which the stem cells are cultured to induce differentiation into cardiomyocytes in the presence of a substance that inhibits BMP signaling.

Method of inducing the differentiation of stem cells into myocardial cells

Growing community of inventors

Ibaraki, Japan

Tomofumi Tanaka