Shionogi Company Limited

Tomoaki Takakura

Akio Takimoto

Kenji Inagaki

Hidehiko Tanaka

Shintaro Misaki

Kensaku Akita

Takaomi Ito

An L-methionine &#x3b3;-lyase having modified function which is obtained by structurally stabilizing natural L-methionine &#x3b3;-lyase by analyzing the structure of crystals thereof by using X-ray, estimating an amino acid sequence concerning its substrate specificity and varying amino acid residue thereof; enzymes comprising a protein constituting the L-methionine &#x3b3;-lyase having modified function or its multimer (preferably its tetramer); DNA sequences encoding these enzymes; a process for producing these enzymes; and medicinal preparations (preferably anticancer agent) containing these enzymes.

L-methionine $g(g)-lyase with modified function

A process for production of L-methionine &ggr;-lyase crystals by using polyethylene glycol, characterized by comprising the first step of warming a solution containing L-methionine &ggr;-lyase before or after addition of polyethylene glycol thereto and the second step of adding an inorganic salt.

Process for producing L-methionine &ggr;-lyase crystals

Growing community of inventors

Amagasaki, Japan

Tomoaki Takakura