Jsr Life Sciences Corporation

Jsr Corporation

Jsr Micro N.v.

Tomoaki Haga

Satoshi Nakamura

Jun-ichi Yasuoka

Takashi Ichii

Kiichi Yoshimura

Shunsuke Onogi

Toshinari Honda

Tomoyuki Kamide

An affinity carrier may have improved alkali resistance. An immunoglobulin-binding protein containing a mutant polypeptide chain, as well as an affinity carrier containing a solid-phase carrier to which the immunoglobulin-binding protein is bound, may be a mutant polypeptide chain having an amino acid sequence having at least 85% identity to an amino acid sequence indicated by any of SEQ ID NO: 1-6 and 57-62, having a predetermined mutation, and having immunoglobulin-binding activity.

Immunoglobulin-binding protein, and affinity carrier using same

Provided are a protein L-derived immunoglobulin binding protein having an increased antibody dissociation rate under acidic conditions, and an affinity support using the same. Disclosed are an immunoglobulin binding protein comprising at least one mutant of an immunoglobulin binding domain, and an affinity support comprising a solid-phase support having the immunoglobulin binding protein bound thereto. A mutant of the immunoglobulin binding domain consists of an amino acid sequence having an identity of at least 85% with the sequence set forth in any one of SEQ ID NO:1 to SEQ ID NO:9 and a predetermined mutation, and the mutant has immunoglobulin κ chain binding activity.

Immunoglobulin binding protein, and affinity support using same

Growing community of inventors

Minato-ku, Japan

Tomoaki Haga