Uniwersytet Jagiellonski

Tomasz Gosiewski

Monika Brzychczy-Wloch

Agata Pietrzyk

Malgorzta Bulanda

Monika Brzychczy-Włoch

An exemplary embodiment describes a method for detection of bacteria and fungi in a sample of biological material, wherein the DNA contained in the sample of biological material is subjected to amplification in multiplex real-time PCR, with the use of primers specific for bacteria in the first stage and primers specific for fungi, and in the second stage, the resulting DNA is amplified using primers and probes differentiating fungi into a group of mold fungi and yeast fungi and bacteria into Gram-positive and Gram-negative bacteria. Another exemplary embodiment refers to oligonucleotide primers for the detection of bacteria and fungi by PCR and a kit for simultaneous detection of bacteria and fungi.

Method for simultaneous detection of bacteria and fungi in a biological preparation by PCR, primers as well as bacteria and fungi detection kit

A method allows the simultaneous isolation of bacterial and fungal DNA from blood. The method uses the enzymatic, mechanical and thermal lysis.

Growing community of inventors

Kraków, Poland

Tomasz Gosiewski