Siemens Aktiengesellschaft

Csem Centre Suisse D'electronique Et De Microtechnique Sa

Eth Zurich, Eth Transfer

Exaddon Ag

Eth Zurich

Cytosurge Ag

Tomaso Zambelli

Janos Vörös

Michael Gabi

Martin Nirschl

Luca Hirt

Pascal Behr

Julia Vorholt-Zambelli

Stefan Pablo Doerig

Kaori Sugihara

Philipp Stiefel

André Meister

Jérôme Polesel-Maris

Stephan Ihle

Janos Voeroes

A method for manufacturing a three-dimensional object comprises the steps of (a) bringing at least one nozzle in a first position close to a surface of a substrate, (b) delivering through said at least one nozzle at least one reactant to said surface, (c) effecting a solid forming reaction of said at least one delivered reactant such that said at least one delivered reactant undergoes a transition to become a growing solid deposit on said surface under said at least one nozzle, and (d) detecting an interaction of said growing solid deposit with said at least one nozzle.

Method for manufacturing a three-dimensional object and apparatus for conducting said method

Small and extremely small molecules and ions or atoms may be detected with the novel device with exceptional sensitivity. The detection is implemented in a simple manner by the known acoustic resonator FBAR or by means of other technologies that measure the physical properties of the filled layer. The permeability of substances (e.g. active ingredients) through membranes such as cell membranes, lipid bilayers, and cell walls can be examined by combining a sensor with the reservoir and the membrane.

Biochip sensor

The invention relates to a probe arrangement () for exchanging in a controllable way liquids with micro-sized samples of material like biological cells, especially in connection with an scanning probe microscope, said probe arrangement () comprising a probe holder () with at least one embedded first channel () and a cantilever () with at least one embedded second channel () and being provided to be attached to said probe holder () in a way that at least one aperture () of the first channel () is connected to at least one aperture () of the second channel () in a way that permits the liquid-tight transfer of a liquid between said first and second channels (). A safe and easy use of the probe arrangement is achieved by having the cantilever () permanently attached to said probe holder () to form a prefabricated probe unit ().

Probe arrangement for exchanging in a controllable way liquids with micro-sized samples of material like biological cells

A probe arrangement with a probe for local electrophysiological analysis of cells () such as patch-clamp techniques for use with atomic force microscopy, has a probe with a cantilever arm () connected to a probe holder (). The probe has a probe tip () at a probing end () of the cantilever arm () and a fluid channel () in the cantilever arm () connecting a probe tip aperture () with a fluid reservoir () via a duct (). The fluid channel (), the duct () and the fluid reservoir () are adapted to be filled with a fluid solution () enabling ion transport for electrophysiological analysis. A first electrode () for electrophysiological analysis is placed in the fluid reservoir () and/or in the duct () and/or in the fluid channel ().

Probe arrangement

Growing community of inventors

Zurich, Switzerland

Tomaso Zambelli