Cytiva Sweden Ab

Tomas Dalmo

Johan Johansson

Per Kjellin

Anna Lager

Lena Vinterbäck

Johan Dan Johansson

The present invention relates to a method for production of an improved sensor surface for an SPR instrument, comprising forming a self assembled monolayer (SAM) on a surface and attaching ligands and protein resistant groups, preferably polyethylene glycol (PEG), directly to functional groups on said surface. The invention also relates to a sensor surface produced by these methods use thereof in SPR (surface plasmon resonance) assays or interactions.

Sensor surface for surface plasmon resonance assays

Methods for preparing a dilution series for use in forming calibration curves, preferably in the field of surface plasmon resonance, are provided. In one example, a dilution series is prepared by using receptacles such as tubes of a micro well plate in which samples of the dilution series are mixed. In another example, a dilution series is prepared by using a convection mix in a receptacle for achieving a concentration gradient in the sample. A biosensor system arranged to perform steps of methods disclosed are provided. A data processing apparatus and a software for performing steps of methods disclosed, and a computer readable medium for storing the software are also provided.

Growing community of inventors

Uppsala, Sweden

Tomas Dalmo