Leland Stanford Junior University

Tom Wehrman

Helen M Blau

Georges Von Degenfeld

Mark Morris Hammer

Methods and compositions for detecting molecular interactions are provided. Aspects of the invention include the use of a reduced affinity enzyme complementation reporter system. Also provided are systems and kits for use in practicing embodiments of the methods.

Detection of molecular interactions using a reduced affinity enzyme complementation reporter system

Methods and compositions for detecting the sub-cellular localization of a molecule are provided. Aspects of the invention include detecting translocation of a cell-surface receptor to a sub-cellular compartment, e.g., the endosome, using a reduced affinity enzyme complementation reporter system. Also provided are systems and kits for use in practicing embodiments of the methods.

Detection of sub-cellular compartment localization of a molecule using a reduced affinity enzyme complementation reporter system

Sequential reporter enzyme luminescence (SRL) methods are provided. In the subject methods, the activity of a reporter enzyme is evaluated using a secondary reporter system that employs a product of a reporter enzyme mediated reaction as a luminescent substrate, e.g., luciferase substrate. Also provided are kits and other compositions that find use in practicing the subject methods.

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Fremont, CA, United States of America

Tom Wehrman