Urit Medical Electronic Co., Ltd.

Life Technologies Corporation

Other

Tom Cheng Xu

Christopher Trinh

Jinlan Xu

Ferrier Le

Shilei Tang

Sally Li Shen

Claire Marjoribanks

Kuikui Shen

Jusong Liang

Yating Shi

Brian Lee Evans

Carole Bornarth

Mousumi Rath

Kathy Tran

Jun Liu

Chengfeng Jiang

Lili Sun

Ferrier Nhat Le

Disclosed herein are methods, reagent kits, and compositions for performing a bisulfite conversion of DNA directly from a biological sample including a patient's urine sample or a slide-mounted FFPE tissue sample. For example, a method of performing a bisulfite conversion of DNA can comprise certain preliminary steps for processing the biological sample and transferring a portion of the processed sample into a reaction vessel containing a bisulfite mixture. The method can further comprise heating the reaction vessel containing the biological sample and the bisulfite mixture at several heating temperatures and subsequently holding the reaction vessel at a holding temperature for a holding period. The method can also comprise certain bisulfite removal steps, desulfonation steps, and removal of the desulfonation solution. A final elution step can yield the converted DNA for further downstream sequencing and analysis.

Methods and reagent kits for bisulfite conversion of DNA

A composition comprising a thermostable DNA polymerase; and a PCR inhibitor blocking agent, wherein the PCR inhibitor blocking agent is present in an amount effective to enhance tolerance of an assembled PCR to a PCR inhibitor.

Compositions, kits and methods for synthesis and/or detection of nucleic acids

Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.

Methods for isolation, identification, and quantification of miRNAs

Disclosed are methods and compositions for preparing a DNA library from slide-mounted FFPE tissue samples for downstream next-generation sequencing. In one embodiment, a method of preparing the DNA library is disclosed. The method can comprise applying a droplet of a reagent mixture onto a slide-mounted FFPE tissue sample. The reagent mixture can comprise one or more buffer solutions, a cofactor, a nonionic surfactant, a glycerol solution, a gelatin solution, dNTPs, a DNA polymerase, and a primer pool comprising a plurality of forward primers and reverse primers. The method can also comprise stirring the droplet in a circular motion on the slide while scraping portions of the FFPE tissue sample mounted on the slide to yield a reaction mixture. The method can further comprise aspirating the reaction mixture from the slide directly into a pipette tip and dispensing the reaction mixture into a reaction vessel for further amplification.

Methods and systems for production of DNA libraries directly from FFPE tissue

Disclosed are methods, compositions, and diagnostic kits for the rapid detection of certain types of &#x3b1;-thalassemia and &#x3b2;-thalassemia. In some embodiments, a diagnostic kit, reagents, and methods are disclosed for the rapid detection of various &#x3b1;-thalassemia and &#x3b2;-thalassemia genotypes in multiple patient samples using real-time PCR. More specifically, in certain embodiments, diagnostic kits, reagents, and methods are disclosed for the rapid detection of up to seven different &#x3b1;-thalassemia genotypes and twenty different &#x3b2;-thalassemia genotypes in one single multiplex real-time PCR reaction.

Methods, compositions, and diagnostic kits for the detection of alpha and beta thalassemia

A fast acting sensor designed to accommodate an aqueous analyte-containing sample having a volume of less than one microliter and that can be used to quantify the amount and concentration of such analyte in the sample through light reflectance or fiber-optic light reflectance. The sensor includes a storage chamber, a capillary passage, and a reaction membrane. The storage chamber acts to collect the sample and to secure such sample while it undergoes detection. The capillary passage acts to direct the sample over the reaction membrane and controls the diffusion of the sample in the storage chamber. The reaction membrane contains all of the chemicals and enzymes needed to cause a color-change reaction when contacted with the sample. The amount of analyte can be determined by light reflectance intensity with an optical measurement instrument.

Small volume and fast acting optical analyte sensor

Growing community of inventors

Castro Valley, CA, United States of America

Tom Cheng Xu