Unchained Labs

Trinean Nv

Tom Boonefaes

A method is described for characterising a sample comprising at least DNA and/or RNA. The method comprises obtaining a UV-VIS spectroscopic measurement of the sample comprising at least DNA and/or RNA, deriving an absorption parameter based on the UV-VIS spectroscopic measurement, and determining a DNA and/or RNA quantification present in the sample. Determining a DNA and/or RNA quantification thereby comprises taking into account a buffer and/or salt effect on the UV-VIS spectroscopic measurement irrespective of the actual buffer and salt content of the sample, based on said derived absorption parameter and reference spectral data including spectral information for buffer and/or salt effects.

Buffer-and-salt-content-independent UV-VIS spectroscopic characterisation of sample DNA and/or RNA content using reference samples having distinct buffer and/or salt content

A method for analyzing UV-VIS spectrophotometer data of a sample comprising at DNA and/or RNA is described. The method comprises receiving UV-VIS spectrophotometer data, fitting the UV-VIS spectrometer data taking into account at least one spectrum representative for a base pair being any of more of adenine-thymine (AT) or guanine-cytosine (GC) or adenine-uracil, and deriving from the fitting a quantification of an amount of DNA and/or RNA.

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Ghent, Belgium

Tom Boonefaes