Ab Bonnierforetagen

Tom B Bjorklund

A process for quantitative determination of the amount of antigen in a solution while using hemagglutination inhibition technique, comprising preparing under serial dilution a series of samples of said solution, adding to each of said samples a predetermined amount of antiserum containing antibodies specific with regard to the antigen in question, adding after incubation to each of the resulting samples a predetermined amount of the antigen carried by a particulate carrier resulting in hemagglutination, and preparing a corresponding series of control samples with known decreasing amounts of antigen. By measuring the diameter of the hemagglutination deposits of the control samples and plotting the measured diameters against the antigen concentrations, an S-shaped curve is obtained, the middle part of which has a steep inclination. By selecting a sample in said series of samples of unknown antigen concentration with a diameter lying within said middle part of the curve, and by means of said diameter, the antigen concentration corresponding thereto can be determined from the curve. Antibody can be measured in a corresponding manner using direct hemagglutination.

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Bromma, Sweden

Tom B Bjorklund