Toyota Jidosha Kabushiki Kaisha

Kabushiki Kaisha Toyota Chuo Kenkyusho

Toyota Jidosha Kabuhsiki Kaisha

Kabuhsiki Kaisha Toyota Chuo Jenjyusho

Tohru Ohnishi

Nobuhiro Ishida

Kenro Tokuhiro

Haruo Takahashi

Satoshi Saitoh

Eiji Nagamori

Masana Hirai

This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).

DNA encoding a protein having D-lactate dehydrogenase activity and uses thereof

The invention provides a promoter that can be used in the presence of an organic acid. The promoter includes DNA with promoter activity of high osmolarity response 7 gene (HOR7 gene), glycelaldehyde 3 phosphate dehydrogenase 2 gene (TDH2 gene), heat shock protein 30 gene (HSP30), hexose transport protein 7 gene (HXT7 gene), thioredoxin peroxidase 1 gene (AHP1 gene), or membrane protein 1 associated gene gene) of yeasts.

Promoter in the presence of organic acid and utilization thereof

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Toyota, Japan

Tohru Ohnishi