Suntory Limited

Kirin Beer Kabushiki Kaisha

Marubeni Kabushiki Kaisha

Tohru Komano

Yuji Shibano

Kazumitsu Ueda

Kanji Ohyma

A yeast host which can express P-glycoprotein, i.e., the product of MDR-related gene, in the cell membrane in the same state as observed in multidrug resistant cells produced by connecting the MDR-related gene which carries multidrug resistance to a yeast expression vector and transforming the yeast with said recombinant vector; a cell membrane fraction containing a substantial amount of P-glycoprotein produced by said yeast and a process for the preparation thereof; and a recombinant vector for expressing the MDR-related gene in a yeast host.

Expression of MDR-related gene in yeast cell

A promoter comprises a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 kbp, which is obtained by cleavage by restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene, said DNA fraction being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L. The promoter is integrated with a DNA having the same sequence as that of DNA derived from a procaryote into a vector.

Growing community of inventors

Kyoto, Japan

Tohru Komano