University of California

Pioneer Hi-Bred International, Inc.

Todd Young

Daniel R Gallie

Robert B Meeley

Timothy George Helentjaris

The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Engineering single-gene-controlled staygreen potential into plants

The present invention is directed to plant genetic engineering. In particular, it is directed to producing green leaves in maize through inhibition of ethylene. The genes involved in producing this phenotype include 1-Aminocyclopropane-1-Carboxylate ('ACC') synthase, ACC oxidase, ACC deaminase, ethylene response sensor ('ERS'), ethylene resistant (&#x201c;ETR&#x201d;), and ethylene insensitive (&#x201c;EIN&#x201d;).

Nucleic acids that encode ERS1 from maize and their uses

The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases, namely ACS2, ACS6, and ACS7 from, are provided. Knockout plant cells having inhibition in expression and/or activity in an ACC synthase such as ACS2, ACS6, or ACS7 and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production are also provided, as are seeds obtained from such plants. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

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The present invention provides new methods of delaying senescence in a plant by inhibiting ACC oxidase, or EIN2 activity in the plant. In particular, it is directed to producing green leaves in maize through inhibition of ethylene. The genes involved in producing this phenotype include ACC deaminase, ethylene response sensor ('ERS'), ethylene resistant ('ETR'), and ethylene insensitive (&#x201c;EIN&#x201d;). The delay in senescence can be achieved through the production of ACC deaminase, mutated ETR1 and ERS2 proteins, as well as overexpression of wild-type ETR1 and ERS2 proteins.

Maize ETR2 gene and uses thereof

The present invention provides new methods of delaying senescence in a plant by inhibiting ACC oxidase, or EIN2 activity the plant. In particular, it is directed to producing green leaves in maize through inhibition of ethylene. The genes involved in producing this phenotype include ACC deaminase, ethylene response sensor ('ERS'), ethylene resistan ('ETR'), and ethylene insensitive (&#x201c;EIN&#x201d;). The delay in senescence can be achieved through the production of ACC deaminase, mutated ETR1 and ERS2 proteins, as well as overexpression of wild-type ETR1 and ERS2 proteins.

Growing community of inventors

Palm Springs, CA, United States of America

Todd Young