E.i. Dupont De Nemours and Company

E. I. Du Pont Nemours and Co.

Todd M Vannelli

Wei Wei Qi

Fateme Sima Sariaslani

Xiao-Song Tang

Zhixiong Xue

Arie Ben-Bassat

Anthony Arthur Gatenby

Sima Sariaslani

Sabine Breinig

An in vivo method for the production of pHS via a recombinant host cell is disclosed. The host cell expresses at least one gene encoding a polypeptide having para-hydroxycinnamic acid decarboxylase activity in combination with either at least one gene encoding a polypeptide having tyrosine ammonia lyase activity or at least one gene encoding a polypeptide having phenylalanine ammonia lyase activity.

Microbial conversion of glucose to para-hydroxystyrene

A novel tyrosine-inducible tyrosine ammonia lyase enzyme was isolated from the yeast. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of para-hydroxycinnamic acid directly from tyrosine. The gene encoding this enzyme was sequenced using 3' and 5&#x2032; RACE cloning of the TAL cDNA and the gene was expressed in the yeastand in the bacterium

DNA and amino acid sequences of a tyrosine-inducible tyrosine ammonia lyase enzyme from the yeast Trichosporon cutaneum

The present invention provides several methods for biological production of para-hydroxycinnamic acid (PHCA). The invention is also directed to the discovery of new fungi and bacteria that possess the ability to convert cinnamate to PHCA. The invention relates to developing of a new biocatalyst for conversion of glucose to PHCA by incorporation of the wild type PAL from the yeast into underlining the ability of the wildtype PAL to convert tyrosine to PHCA. The invention is also directed to developing a new biocatalyst for conversion of glucose to PHCA by incorporation of the wildtype PAL from the yeast plus the plant cytochrome P-450 and the cytochrome P-450 reductase into . In yet another embodiment, the present invention provides for the developing of a new biocatalyst through mutagenesis of the wild type yeast PAL which possesses enhanced tyrosine ammonia-lyase (TAL) activity.

Bioproduction of para-hydroxycinnamic acid

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Ithaca, NY, United States of America

Todd M Vannelli