Georgia Tech Research Corporation

University of California

The J. David Gladstone Institutes, a Testamentary Trust Established Under the Will of J. David Gladstone

Todd Christopher McDevitt

Andres Jose Garcia

Hang Lu

Ankur Singh

Shalu Suri

Rehka Ramachandran Nair

Jessica Butts

Methods of generating spinal cord glutamatergic interneurons (V2a interneurons) from human pluripotent stem cells (hPSCs) are provided. A method of the present disclosure may include culturing a first population of hPSCs in vitro in a neural induction medium that includes: a retinoic acid signaling pathway activator; a sonic hedgehog (Shh) signaling pathway activator; and a Notch signaling pathway inhibitor, wherein the culturing results in generation of a second population of cultured cells containing CHX10+ V2a interneurons. Also provided are non-human animal models that include the hPSC-derived spinal cord glutamatergic interneurons, and methods of producing the non-human animal models.

In vitro methods of differentiating stem cells into neurons and neurons generated using the same

The present invention provides for methods of isolating a stem cell or cell derived therefrom from a mixture of cells, for example, a mixture of adherent cells in culture. Cell isolation is achieved by the application of selective detachment forces.

Adhesive signature-based methods for the isolation of stem cells and cells derived therefrom

Compositions containing acellularized biomaterial derived from differentiating pluripotent cells, for example, embryonic stem cells are provided. The acellularized biomaterial can be used to promote wound healing, promote tissue regeneration, or inhibit scarring. Methods for using the acellularized biomaterial for treating degenerative diseases are also provided.

Acellularized biomaterial from embryonic stem cells

Growing community of inventors

Atlanta, GA, United States of America

Todd Christopher McDevitt