Midwest Research Institute

Alliance for Sustainable Energy, LLC

Todd B Vinzant

Michael E Himmel

William S Adney

Shi-You Ding

Stephen Robert Decker

John O Baker

Steven Slayzak

Peter N Ciesielski

Ren S Anderson

Daniel M Blake

Suzanne Lantz McCarter

Suzanne Mc Carter

Raphael Nieves

Ronald D Judkoff

Shih-You Ding

Joseph P Ryan

The present disclosure relates to a composition that includes a base layer having an outer surface and a first thickness and a material that includes a plurality of cellulose nanofibers in physical contact with the outer surface, where the composition has an average filtration efficiency of less than or equal to 90% for particles having a characteristic length between about 50 nm and about 100 μm and the composition is characterized by an average inhalation resistance of less than or equal to 35 mm HO as measured across the first thickness and the material.

Cellulose-containing filtration materials and methods of making the same

The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from, and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

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The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized fromand, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

Thermal tolerant avicelase from Acidothermus cellulolyticus

The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as Gux1. Gux1 has been isolated and characterized from. The invention further provides recombinant forms of the identified Gux1. Methods of making and using Gux1 polypeptides, including fusions, variants, and derivatives, are also disclosed.

The disclosure provides a method for preparing an active exoglucanase in a heterologous host of eukaryotic origin. The method includes mutagenesis to reduce glycosylation of the exoglucanase when expressed in a heterologous host. It is further disclosed a method to produce variant cellobiohydrolase that is stable at high temperature through mutagenesis.

Cellobiohydrolase I gene and improved variants

A method, and systems for implementing such method, for purifying and conditioning air of weaponized contaminants. The method includes wetting a filter packing media with a salt-based liquid desiccant, such as water with a high concentration of lithium chloride. Air is passed through the wetted filter packing media and the contaminants in are captured with the liquid desiccant while the liquid desiccant dehumidifies the air. The captured contaminants are then deactivated in the liquid desiccant, which may include heating the liquid desiccant. The liquid desiccant is regenerated by applying heat to the liquid desiccant and then removing moisture. The method includes repeating the wetting with the regenerated liquid desiccant which provides a regenerable filtering process that captures and deactivates contaminants on an ongoing basis while also conditioning the air. The method may include filtration effectiveness enhancement by electrostatic or inertial means.

Using liquid desiccant as a regenerable filter for capturing and deactivating contaminants

The invention provides a thermal tolerant mannanase that is a member of the glycoside hydrolase family. The invention further discloses this mannanase as ManA. ManA has been isolated and characterized from. The invention further provides recombinant forms of the identified ManA. Methods of making ManA polypeptides, including fusions, variants, and derivatives, are also disclosed. Methods of using mannanase A, including for the processing of food and for use in food stuffs as bulking agents and the like, are also disclosed.

Thermal tolerant mannanase from acidothermus cellulolyticus

The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as GuxA. GuxA has been isolated and characterized fromThe invention further provides recombinant forms of the identified GuxA. Methods of making and using GuxA polypeptides, including fusions, variants, and derivatives, are also disclosed.

A process for production of an alkaline tolerant dextranase enzyme comprises culturing a dextran-producing microorganism having accession no. ATCC PTA-3866 to produce an alkaline tolerant dextranase, Dex 1 wherein the protein in said enzyme is characterized by a MW of 63.3 kDa and Dex 2 wherein its protein is characterized by a MW of 81.8 kDa.

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Golden, CO, United States of America

Todd B Vinzant