California Institute of Technology

Massachusetts Institute of Technology

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Todd A Thorsen

Stephen R Quake

Axel Scherer

Hou-Pu Chou

Marc Alexander Unger

Carl L Hansen

Mark L Adams

Markus M Enzelberger

Vincent Studer

Jian Liu

Jeremy Bert Muldavin

Sebastian J Maerkl

Xiaoyan Bao

Rahul Sarpeshkar

Julia A Kornfield

Carl O Bozler

Martin Arnold Schmidt

Jakub Tadeusz Kedzierski

Donna-Ruth Webb Yost

Martin Zdenek Bazant

Benjamin I Rapoport

Jeremy A Levitan

Reuel Bennett Swint

Marc Ferrer-Alegre

Boris G Kharas

Johanna Bobrow

Min Jae Song

Yen-Ting Tung

Michale Sean Fee

Raoul Ouatagom Ouedraogo

David Walsh

Sam Michael

Christina Zook

Eric Phelps

Molly Elizabeth Boutin

Christopher Cherry

Gregory D Allen

Mark A Unger

Rob G H Lammertink

An in vitro tissue plate may include a well plate, a fluidic plate disposed on a bottom surface of the well plate, and a media manifold disposed on a bottom surface of the fluidic plate. The well plate may have at least two wells, including a tissue well and a waste well. The fluid plate may include a fluid channel extending between and fluidly connecting the tissue well to the waste well. The media manifold may include a one or more media outlets fluidly connected to the fluid channel. A tissue layer may be deposited in the tissue well. The tissue layer may include human cells such as neurovascular cells.

In vitro tissue plate

The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.

Apparatus and methods for conducting assays and high throughput screening

Miniature multifunctional antennas and related techniques are disclosed that are capable of wide bandwidth operation. In some embodiments, the antennas are capable of being reconfigured in the field for optimal performance in different frequency band configurations (e.g., a single wide instantaneous bandwidth, multiple smaller bands, etc.) and/or for purposes of self healing. In some embodiments, the antennas can be reconfigured in the field to achieve different polarizations (e.g., vertical, horizontal, circular). The antennas can be implemented in a very compact manner making them ideal for use in devices and platforms where size and weight are a concern.

Miniature ultra-wideband multifunctional antennas and related techniques

Electrical and electromechanical devices often require maintaining a specific temperature, or a narrow range or temperatures, during operation. An assembly regulates the temperature of a device by providing a variable thermal resistance between the device and a heatsink. The device can be mounted to a base having a high thermal resistance, the base thermally isolating the device from the heatsink. At low environmental temperatures, the base enables the device to rise to its operating temperature as a result of the device's waste heat, and with no or minimal use of a heater. As the environmental temperature increases, a working fluid, having a low thermal resistance, undergoes thermal expansion to fill a portion of a volume in the base between the device and the heat sink, lowering the thermal resistance between the device and the heatsink to maintain the device at the required operating temperature.

Variable thermal resistance

A glucose fuel cell for reception into a given constrained volume of implantation in a vertebrate in which the glucose fuel cell has access to fluid containing glucose. The fuel cell includes an anode adapted to oxidize the glucose, a cathode adapted to reduce an oxidant, and a membrane disposed between the anode and the cathode and separating the anode from the cathode. At least one of the anode or cathode define a flexible sheet that is geometrically deformed to be receivable into the given constrained volume of implantation and increase volumetric power density. Related methods of making a glucose fuel cell of this type and implantable assemblies including the glucose fuel cell are also disclosed.

Flexible and implantable glucose fuel cell

High-density microfluidic chips contain plumbing networks with thousands of micromechanical valves and hundreds of individually addressable chambers. These fluidic devices are analogous to electronic integrated circuits fabricated using large scale integration (LSI). A component of these networks is the fluidic multiplexor, which is a combinatorial array of binary valve patterns that exponentially increases the processing power of a network by allowing complex fluid manipulations with a minimal number of inputs. These integrated microfluidic networks can be used to construct a variety of highly complex microfluidic devices, for example the microfluidic analog of a comparator array, and a microfluidic memory storage device resembling electronic random access memories.

Microfabricated structure having parallel and orthogonal flow channels controlled by row and column multiplexors

A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Microfabricated elastomeric valve and pump systems

A microfluidic device is provided for analyzing or sorting biological materials, such as polynucleotides, polypeptides, proteins, enzymes, viruses and cells. The invention can be used for high throughput or combinatorial screening. The device comprises a main channel and an inlet channel that communicate at a droplet extrusion region so that droplets of solution are deposited into an immiscible solvent in the main channel. Droplets can thereafter be sorted according to biological material detected in each droplet.

Microfabricated crossflow devices and methods

This invention provides microfabricated devices and methods for detecting, analyzing and sorting biological materials and particles. Droplets containing the particles are provided in an extrusion fluid, passed through a detection region, and then directed into a branch channel according to predetermined characteristics. For example, cells or viral particles contained in droplets of aqueous solvent are flowed past a detector in the nonpolar extrusion fluid decane, and routed into a selected branch channel for subsequent analysis or use.

Growing community of inventors

Pasadena, CA, United States of America

Todd A Thorsen