New England Biolabs, Inc.

Toby E Claus

Richard D Morgan

Romualdas Vaisvila

Rebecca B Kucera

Elisabeth A Raleigh

Jack S Benner, Ii

A method for cloning restriction-modification system is provided whereby the target modification methylase is produced and confers full protection during all growth phases in which the cognate restriction enzyme is present. The method is employed in the cloning of the MseI restriction-modification system.

Method for cloning and producing the Msel restriction endonuclease

The present invention is directed to a method for cloning and producing the NotI restriction endonuclease by 1) introducing the restriction endonuclease gene from Nocardia otitidis-caviarum into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity, and 3) purifying the NotI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity.

Growing community of inventors

Beverly, MA, United States of America

Toby E Claus