Becton Dickinson and Company Limited

Tobin J Hellyer

James G Nadeau

Ray A McMillian

Thomas L Fort

Keith Edward Thornton

Dolores M Berger

William A Nussbaumer

James A Price, Jr

Jianrong Lou

Robert W Rosenstein

Sha-Sha Wang

Andrew Kuhn

Qimin You

David M Wolfe

Daretta A Yursis

Lisa M Keller

Benoit Dore

Christian Menard

James M Harris

Donald W Copertino

Panayiotis Zagouras

Karen Eckert

Erika L Jones

Sha Sha Wang

Stefanie M Finn

Janet L Wood

Thomas L Brink

Christing A Rostkowski

Jim Nadeau

Keith Thornton

Robert Rosenstein

Ray A Mcmillian

Nucleic acid amplification primers and methods for specific detection of influenza A and influenza B nucleic acid targets are disclosed. The primer-target binding sequences are useful for detection of influenza A and influenza B targets in a variety of amplification and hybridization reactions. The oligonucleotide sequences are able to differentiate between influenza A and influenza B strains through specific hybridization to one or the other virus strain, enabling specific detection of the presence of influenza A and/or influenza B in a specimen.

Sequences and methods for detecting influenza A and influenza B virus

A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

Immuno-amplification

A full process control for use with a molecular assay and a method of determine the efficacy of the molecular assay. A full process control can include a fixed cell, and specifically can include a fixed vegetative cell. A method of determining the efficacy of a molecular assay can include providing an internal control, mixing the internal control with a sample, lysing the internal control and the sample, and detecting the lysis product. The full process control and/or the internal control can becells.

Process controls for molecular assay

Oligonucleotides for detection of nucleic acid in a sample that provide for an amplified signal by recycling probes and probe fragments.

Assay method for target nucleic acid by signal amplification using probe hybridization and restriction

The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.

Use of an extraction control in a method of extracting nucleic acids

The present invention relates, in general, to probes, methods, and kits used to determine the presence or absence of a microorganism in a sample. The probes, methods, and kits comprise at least one capture probe and/or at least one detector probe.

Method for rapid identification of microorganisms

The invention employs an unlabeled signal primer comprising a 5' adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3&#x2032; end of which hybridizes to the complement of the 5&#x2032; adapter sequence of the signal primer to produce a 5&#x2032; overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5&#x2032; overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

Methods for detecting nucleic acid sequence variations

Primers and probes derived from SARS-CoV nucleic acid that facilitate detection and/or quantification of the replicase gene are disclosed. The disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of SARS-CoV infection.

Assay for SARS coronavirus by amplification and detection of the replicase sequence

Primers and probes derived from the HIV-1 pol gene which facilitate detection and/or quantification of all presently known genotypes of HIV-1 (A-I and O). Disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of HIV nucleic acids.

Sequences for detection of HIV-1

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Owings Mills, MD, United States of America

Tobin J Hellyer