Wacker Chemie Ag

Consortium Fur Elektrochemische Industrie Gmbh

Tobias Dassler

Gerhard Schmid

Guenter Wich

Anneliese Reutter-Maier

Thomas Schloesser

Thomas Maier

Walfred Leinfelder

Markus Brunner

Marian Kujau

August B&ouml;ck

Simone Mitterweger

Kerstin Pfeiffer-Schwiesow

Günter Wich

Thomas SCHLÖSSER

Anbacterial strain contains a gene encoding a recombinant protein and an open reading frame of

Bacterial lpp mutants and the use thereof for the secretory production of recombinant proteins

The invention relates to a method for producing L-cysteine and its derivatives L-cystine and thiazolidine by fermenting a cysteine-producing microorganism strain in a fermentation medium. The invention is characterized in that L-methionine, L-isoleucine, or L-threonine in respective concentrations ranging from 0.1 to 10 g/L are added to the fermentation medium in the main culture.

Method for the fermentative production of L-cysteine and derivatives of said amino acid

The invention relates to a method for producing L-cystine by fermenting a microorganism strain in a fermentation medium, in which method L-cystine is precipitated in an amount of at least 70% relative to the total cysteine, characterized in that the Osaturation of the fermentation medium is kept at least at 1% and at most at 40&#xb1;3% during the formation of L-cystine.

Method for producing L-cystine by fermentation under controlled oxygen saturation

A method for the production of natural L-cysteine by fermentation in a production fermenter in which a microorganism strain is cultured in a fermentation medium, characterized in that the fraction of the compounds L-cysteine, L-cystine and thiazolidine in the fermentation medium is controlled in a targeted manner by an iron concentration of a maximum of 8 mg/l in the fermentation medium.

Method for production of natural L-cysteine by fermentation

The present invention relates to a process for producing a correctly folded and assembled full-length antibody using anstrain, which comprises fermenting anstrain which leaks periplasmic proteins into the medium, comprising a gene coding for the heavy chain of an antibody functionally linked to a signal sequence coding for a signal peptide, and a second gene coding for the light chain of an antibody, functionally linked to a signal sequence coding for a signal peptide, in a culture medium, where thestrain secretes a full-length antibody into the culture medium, and the full-length antibody is removed from the culture medium.

Process for the fermentative production of antibodies

A process for producing a heterologous protein is provided. The process comprises culturing anstrain in a fermentation medium, thestrain harboring a gene which codes for the heterologous protein and is functionally linked to a signal sequence coding for a signal peptide, and releasing the heterologous protein into the fermentation medium, and the heterologous protein being removed from the fermentation medium, wherein thestrain has an attenuated (p)ppGpp-synthetase II activity (PSII activity).

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A DNA construct that allows the inexpensive production of a target protein in, consisting of a nucleic acid sequence encoding a signal peptide which is operably linked with a gene coding for a carrier protein which is linked with a gene coding for the target protein via a gene coding for a cleavable sequence S, wherein the gene coding for a carrier protein is the spy gene from

DNA construct and process for the fermentative production of fusion proteins

A process is provided for the fermentative preparation of O-acetyl-L-serine. A microorganism strain, which is derived from a wild type and which exhibits an increased endogenous formation of O-acetyl-L-serine and an increased efflux of O-acetyl-L-serine as compared with the wild type, is cultured in a fermentation medium. A pH in the range from 5.1 to 6.5 is set in the fermentation medium.

Growing community of inventors

Munich, Germany

Tobias Dassler