10x Genomics, Inc.

Caliper Life Sciences, Inc.

University of Illinois

Washington University

Cornell University

Tobias Daniel Wheeler

Rajiv Bharadwaj

Mohammad Rahimi Lenji

Katherine Pfeiffer

Andrew D Price

Christopher Hindson

Anthony Makarewicz

Bill Kengli Lin

Yifeng Yin

Kevin D Ness

Michael Schnall-Levin

Pranav Patel

Zahra Kamila Belhocine

Jessica Michele Terry

Phillip Belgrader

Benjamin J Hindson

Serge Saxonov

John R Stuelpnagel

Bradley W Rice

Daniel G Stearns

Michael Ybarra Lucero

Tarjei Sigurd Mikkelsen

Geoffrey McDermott

Zachary Bent

Paul Ryvkin

Josephine Harada

Elliott Meer

Niranjan Srinivas

Indira Wu

Vijay Kumar Sreenivasa Gopalan

Stephane Claude Boutet

Solongo B Ziraldo

I-Jane Chen

Christopher Joachim O'Keeffe

Joshua I Molho

Sarah Taylor

Danh C Tran

Kamila Belhocine

Josephine Lee

Paul J A Kenis

David Sukovich

Francesca Meschi

Alexander V Dukhovny

Abraham D Stroock

Dexing Zeng

Luz Montesclaros

Alireza Salmanzadeh

Jerald Sapida

Amit V Desai

David E Reichert

Martin Sauzade

Yiran Zhang

Birce C Önal

Ivan Akhremichev

Lynna Chen

The present disclosure provides methods and systems for nucleic acid processing. A method for preparing a sequencing set may include providing a template nucleic acid and amplifying the template nucleic acid to provide a complementary nucleic acid. Next, the complementary nucleic acid may be fragmented and barcoded to produce a first set of barcoded fragments comprising a plurality of first barcoded fragments. Next, the plurality of first barcoded fragments may be fragmented to yield a second set of barcoded fragments comprising a plurality of second barcoded fragments.

Systems and methods for nucleic acid preparation

Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

Devices, systems, and methods for increasing droplet formation efficiency

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Methods and systems for analysis

Methods and systems for droplet-based single cell barcoding

Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

Microfluidic systems and methods of use

Provided herein are methods, compositions, and systems for multiplexed analysis of individual cells or cell populations. Cells encapsulated in beads and/or biomolecules are sequentially co-partitioned, allowing for analysis of two different types of biomolecules (e.g., RNA and DNA). The present invention leverages different polymer dissociation mechanisms, accompanied with barcoding of biomolecules (e.g., nucleic acid molecules) for multiplexed measurements in single cells. Sequential co-partitioning and barcode technology enables identification and quantitation of DNA and RNA from single cells.

Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

Microfluidic channel networks for partitioning

The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

Methods and systems for sample preparation and analysis

A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

Microfluidic surface-mediated emulsion stability control

A microfluidic system includes a microfluidic chip having a plurality of fluid channels, each fluid channel having an opening providing access to an interior of the fluid channel, and a gasket disposable on the microfluidic chip in an aligned configuration, the gasket including a first side configured to face the microfluidic chip in the aligned configuration, a second side opposite the first side, and an aperture extending through the gasket from the first side to the second side, the aperture being sized and positioned to allow a communication of pressure from the second side of the gasket to the openings of at least two fluid channels when the gasket is in the aligned configuration.

Gaskets for the distribution of pressures in a microfluidic system

Growing community of inventors

Alameda, CA, United States of America

Tobias Daniel Wheeler