Tokitae LLC

Toan Huynh

Bernhard Hans Weigl

Kevin Paul Flood Nichols

Samantha A Byrnes

Joshua Davis Bishop

Benjamin David Grant

Damian Madan

Caitlin E Anderson

David Michael Cate

An embodiment of a system includes a compartment-generating device, a compartment detector, and electronic computing circuitry. The device is configured to generate compartments of a digital assay, at least one of the compartments having a respective volume that is different from a respective volume of each of at least another one of the compartments. The detector is configured to determine a number of the compartments each having a respective number of a target that is greater than a threshold number of the target. And the electronic circuitry is configured to determine a bulk concentration of the target in a source of the sample in response to the determined number of compartments. Because such a system can be configured to estimate a bulk concentration of a target in a source from a polydisperse digital assay, the system can be portable, and lower-cost and faster, than conventional systems.

Determining a bulk concentration of a target in a sample using a digital assay with compartments having nonuniform volumes

Embodiments include assay devices within a single use, disposable cassette for biochemical assays with at least one fluid well and at least one capillary valve. The capillary valve in combination with motion of porous sheets within the assay leads to addition of liquid reagents at different times to the assay although a user adds the liquid reagents to the cassette simultaneously.

Assay structures for multi-step biochemical assays

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Seattle, WA, United States of America

Toan Huynh