Lanthiopep B.v.

Applied Nanosystems B.v.

Morphosys Ag

Tjibbe Bosma

Josef Prassler

Cornelis Johannes Leenhouts

Rick Rink

Gert Nikolaas Moll

Markus Moosmeier

Maarten Leonardus Van Roosmalen

Johannes Herbert Urban

Markus Andreas Moosmeier

Maarten Leonardus Van Rosmalen

The present invention relates to methods for displaying cyclic peptides on the surface of bacteriophage particles and collections thereof.

Methods for displaying cyclic peptides on bacteriophage particles

The invention relates to bacterial cell surface display of post-translationally modified heterologous proteins. Provided is an isolated nucleic acid construct encoding a proteinaceous substance comprising, from the N-terminus to the C-terminus, at least (a) an N-terminal a lantibiotic leader sequence; (b) an amino acid sequence of interest to be post-translationally modified to a dehydroresidue- or thioether-bridge containing polypeptide; (c) a hydrophilic cell-wall spanning domain; (d) a sortase recognition motif; (e) a hydrophobic membrane spanning domain and (f) a C-terminal charged membrane anchoring domain. Also provided is a Gram-positive host cell expressing the construct, as well as a library of host cells.

Bacterial surface display and screening of thioether-bridge-containing peptides

The invention relates to the areas of immunology and vaccine delivery. More specifically, it relates to a bacterial vaccine delivery technology with built-in immunostimulatory properties which allows the immobilization of any antigen of interest, without prior antigen modification. Provided is an antigen-loaded immunogenic carrier complex comprising at least one bifunctional polypeptide attached to an immunogenic carrier, said bifunctional polypeptide comprising a peptidoglycan binding domain (PBD) through which the polypeptide is attached to said carrier, fused to an antigen binding domain (ABD) to which at least one antigen of interest is bound. Also provided is a pharmaceutical (e.g. vaccine) composition comprising an antigen-loaded immunogenic carrier complex of the invention.

Bifunctional protein anchors

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Lippenhuizen, Netherlands

Tjibbe Bosma