Novo Nordisk A/s

Novozymes A/s

Novo Nordisk Biotech, Inc.

Novozyme A/s

Titus Kretzschmar

Jesper Vind

Torben Vedel Borchert

Joel R Cherry

Allan Svendsen

Kim Borch

Shamkant Anant Patkar

Torben Halkier

Claus Crone Fuglsang

John C Royer

Marianne Thellersen

Steen Troels Jorgensen

Jens Sigurd Okkels

Dorte Aaby Petersen

Vind Jesper

The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

Lipolytic enzymes

The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

A method for the construction of a library of recombined polynucleotides from a number of different starting single or double stranded parental DNA templates is disclosed, wherein the starting single or double stranded parental DNA templates represent discrete points in a population of genes encoding evolutionary or synthetic homologues of a peptide having homologies ranging over a broad spectrum from less than 15% to more than 80%, said population exhibiting at least one identification sequence, and whereby said genes are subjected to a gene shuffling procedure to generate shuffled mutants of said population of genes representing additional discrete points between those of said starting templates.

Methods of producing polynucleotide variants

The present invention relates to a new method of shuffling especially heterologous polynucleotide sequences, screening and/or selection of new recombinant proteins resulting therefrom having a desired biological activity, and especially to production and identification of novel proteases exhibiting desired properties.

Shuffling of heterologous DNA sequences

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Vaerloese, Denmark

Titus Kretzschmar