Pioneer Hi-bred International, Inc.

Monsanto Technology LLC

Tishu Cai

Zuo-Yu Zhao

Dorothy A Pierce

Michael T Mann

Yuechun Wan

David D Songstad

Anita Otal

Shubha Subbarao

Weining Gu

Richard A Yingling

Laura Ann Tagliani

The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.

Methods for corn transformation

The present invention relates to methods for improving the transformation frequency of -mediated transformation of maize embryos. A preferred method for transforming maize using comprises the steps of: contacting at least one immature embryo from a maize plant with capable of transferring at least one gene to said embryo; co-cultivating the embryos with culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

Methods for Agrobacterium-mediated transformation

Methods and compositions for the efficient transformation of sorghum is provided. The method involves infection with Agrobacterium, particularly those comprising a super-binary vector. In this manner, any gene of interest can be introduced into the sorghum plant. The transformed gene will be flanked by at least one T-DNA border and present in the transformed sorghum in low copy number. Transformed sorghum, cells, tissues, plants, and seed are also provided. The invention encompasses regenerated, fertile sorghum plants, transgenic seeds produced therefrom, T1 and subsequent generations.

Agrobacterium mediated transformation of sorghum

The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

Methods for agrobacterium-mediated transformation

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Ellisville, MO, United States of America

Tishu Cai