Colgate Palmolive Company

Abelzeta Inc.

Shanghai Cellular Biopharmaceutical Group Ltd.

Shanghai Abelzeta Ltd.

Ting Yan

Wen Jin Xi

Bo Zhang

Fang Liu

Guang Sheng Guo

Haojie Zhang

Jiangguo Ying

Yi Hong

Luyi Zhang

Hao Qing

Yanmei Ji

Fei Wang

Yan Mei Ji

Wenjin Xi

Dijun Zhao

Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (l) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Method for large-scale production of lentivirus by using GMP-level serum-free suspension cells

Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Method for large-scale preparation of purified preparation of recombinant lentiviral vector at GMP grade

Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (1) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

An oral care implement including at least one multi-component bristle. In one aspect, the invention can be an oral care implement having a handle; a head coupled to the handle; at least one bristle tuft extending from the head, the at least one bristle tuft comprising at least one multi-component bristle comprising first and second components. The first component may include a first plastic and a first oral care additive and the second component may include a second plastic and a second oral care additive such that the first oral care additive is different than the second oral care additive.

Multi-component bristle having components with different oral care additives, and oral care implement comprising the same

An oral care implement () including at least multi-component bristle. The oral care implement () comprises a handle (); a head () coupled to the handle (); at least one bristle tuft extending from the head, the at least one bristle tuft comprises at least one multi-component bristle () comprising coextruded core () and sheath components (), the sheath component () surrounding a first portion () of the core component (), and a second portion () of the core component () protruding from the sheath component () at a tip portion () of the multi-component bristle (); the sheath component () comprises a first plastic and a first oral care additive; the core component () comprises a second plastic and a second oral care additive, wherein the first oral care additive is different than the second oral care additive.

Oral care implement

An oral care implement including at least one spiral bristle. In one aspect, the invention can be an oral care implement having a handle; a head coupled to the handle; at least one bristle tuft extending from the head, the at least one bristle tuft comprising at least one spiral bristle comprising coextruded first and second strand components that are intertwined together to form the at least one spiral bristle; the first strand component comprising a first plastic and a first oral care additive; and the second strand component comprising a second plastic and a second oral care additive, Tine rein the first oral care additive is different than the second oral care additive.

Spiral bristle having strand components with different oral care additives, and oral care implement comprising the same

An oral care implement with a gripping portion. The oral care implement is a toothbrush () including an elongated body () formed of a first material and a handle () including a proximal gripping portion () and a distal neck portion () supporting a head () containing a plurality of tooth cleaning elements (). A longitudinally elongated through slot () is formed in the gripping portion () of the handle () which extends transversely between front and rear exposed surfaces () of the handle (). A second material which is elastomeric is disposed in the slot (). A longitudinally elongated depression () having a transversely concave profile is formed in the rear exposed surfaces () of the handle () which communicates with the slot (). The second material extends at least partially in the depression ().

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Shanghai, China

Ting Yan