Amazon Technologies, Inc.

University of Southern California

Timothy Wells Hunt

Scott E Fraser

Rahul Sharma

Sanjeev Krishnan

Jason Junge

Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

Optimized gene editing utilizing a recombinant endonuclease system

Systems and methods are described for management of data transmitted between computing devices in a communication network. An administrative component can configure one or more devices in the communication path of messages to be exchanged by devices to interpret codes embedded in the communication messages. A receiving device can review incoming messages for one or more processing codes or instructions that are embedded in the portion of the communication typically utilized solely to identify the subject matter of the communication, generally referred to as the topic portion of the communication. The receiving devices can then process the embedded codes to determine how the communication message will be routed or otherwise processed.

Growing community of inventors

Seattle, WA, United States of America

Timothy Wells Hunt