Pioneer Hi-bred International, Inc.

Other

Pioneer Hi-bred, Inc.

Timothy W Fox

Mary Trimnell

Yongzhong Wu

Carl W Garnaat

Marc C Albertsen

Howard P Hershey

Gary A Huffman

Timmy L Kendall

Andrew Mark Cigan

Benjamin A Bowen

Terry EuClaire Meyer

Robert B Meeley

John A McElver

Erica Unger

John A Arbuckle

Terry Euclaire Meyer

Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes.

Dominant gene suppression transgenes and methods of using same

A promoter isolated from, designated the P67 promoter, provides a high level of specificity for expression in developing pollen, particularly at the mid-uninucleate stage, as confirmed by RT-PCR and Northern blot analyses of RNA samples from various tissues. Compositions and methods of use of the P67 promoter are disclosed.

Maize promoter P67 and methods of use

A promoter isolated from, designated the P95 promoter, provides a high level of specificity for expression in developing pollen, particularly at the mid-uninucleate stage, as confirmed by RT-PCR and Northern blot analyzes of RNA samples from various tissues. Compositions and methods of use of the P95 promoter are disclosed.

Maize promoter P95 and methods of use

Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of plants generated or maintained by the methods, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are regulatory elements for pollen-preferred expression of linked polynucleotides. Also provided are methods for identifying gene function, and methods for repressing transmission of transgenes.

The present invention relates to isolated male tissue-preferred regulatory sequences from Ms45 gene. The invention also relates to the use of Ms45 gene male tissue-preferred regulatory sequences in mediating male fertility in a plant, and in producing hybrid seed. The Ms45 male tissue-preferred regulatory region can be operably linked to exogenous genes encoding cytotoxins or inhibitory molecules. The invention also relates to plant cells, plant tissue and plants that contain the MS45 gene regulatory sequences.

Male tissue-preferred regulatory sequences of Ms45 gene and method of using same

A method for the rapid isolation and identification of the DNA sequence of transposable element-tagged genes is provided. The method comprises a modified AFLP approach using a transposable element-anchored amplification to identify and clone an amplification product that is associated with a mutant phenotype. Once cloned, the amplification product of interest may be used to screen a cDNA library directly or may be sequenced and compared to available databases for sequence homology. A modification of this approach provides a method for the identification of the location of an additional type of insertion event into genomic DNA, more specifically transgene insertion into the genome of a host organism.

Transposable element-anchored, amplification method for isolation and identification of tagged genes

The present invention relates to an isolated nucleic acid sequence encoding the Ms45 male tissue-preferred regulatory region. In one aspect this invention relates the use of this male tissue-preferred regulatory region in mediating fertility. An example of such use is the production of hybrid seed such as in a male sterility system. The Ms45 male tissue-preferred regulatory region can be operably linked with exogenous genes, such as those encoding cytotoxins, complementary nucleotidic units and inhibitory molecules. This invention also relates to plant cells, plant tissue and differentiated plants which contain the regulatory region in this invention.

Male tissue-preferred regulatory region and method of using same

Nucleotide sequences encoding a maize 5C9 polypeptide, the 5C9 polypeptide and structural and functional equivalents, and methods for controlling insect infestation in plants are provided.

Growing community of inventors

Des Moines, IA, United States of America

Timothy W Fox